Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 521943..522561 Replicon chromosome
Accession NZ_CP028117
Organism Escherichia coli O111 str. RM9322

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I3O_RS02600 Protein ID WP_001291435.1
Coordinates 521943..522161 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I3O_RS02605 Protein ID WP_000344800.1
Coordinates 522187..522561 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3O_RS02565 517232..517804 + 573 WP_000779826.1 lipoprotein -
I3O_RS02570 517835..518146 - 312 WP_000409911.1 MGMT family protein -
I3O_RS02580 518525..518878 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I3O_RS02585 518920..520470 - 1551 WP_001369170.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I3O_RS02590 520634..521104 - 471 WP_001345013.1 YlaC family protein -
I3O_RS02595 521220..521771 - 552 WP_000102564.1 maltose O-acetyltransferase -
I3O_RS02600 521943..522161 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I3O_RS02605 522187..522561 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I3O_RS02610 523107..526256 - 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
I3O_RS02615 526279..527472 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T100559 WP_001291435.1 NZ_CP028117:c522161-521943 [Escherichia coli O111 str. RM9322]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T100559 NZ_CP028117:c522161-521943 [Escherichia coli O111 str. RM9322]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT100559 WP_000344800.1 NZ_CP028117:c522561-522187 [Escherichia coli O111 str. RM9322]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT100559 NZ_CP028117:c522561-522187 [Escherichia coli O111 str. RM9322]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References