Detailed information of TA system TA100554

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5532601..5533433
• Replicon: chromosome
• Accession: NZ_CP028116
• Organism: Escherichia coli O26 str. RM8426

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: I3M_RS30565
• Protein ID: WP_000854753.1
• Coordinates: 5533059..5533433 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: I3M_RS30560
• Protein ID: WP_001315620.1
• Coordinates: 5532601..5532969 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3M_RS30525	5529437..5529892	+	456	WP_000581504.1	hypothetical protein	-
I3M_RS30530	5529971..5530204	+	234	WP_001119729.1	DUF905 family protein	-
I3M_RS30540	5530304..5531122	+	819	WP_001234622.1	DUF945 domain-containing protein	-
I3M_RS30545	5531177..5531662	+	486	WP_000849588.1	antirestriction protein	-
I3M_RS30550	5531678..5532154	+	477	WP_001186774.1	RadC family protein	-
I3M_RS30555	5532217..5532438	+	222	WP_000692345.1	DUF987 domain-containing protein	-
I3M_RS30560	5532601..5532969	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I3M_RS30565	5533059..5533433	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
I3M_RS30570	5533430..5533918	+	489	WP_000777547.1	hypothetical protein	-
I3M_RS30575	5533930..5534127	+	198	WP_000839281.1	DUF957 domain-containing protein	-
I3M_RS31915	5534212..5534364	+	153	WP_001280445.1	hypothetical protein	-
I3M_RS30600	5535409..5536059	+	651	WP_001037966.1	HNH endonuclease	-
I3M_RS30605	5536067..5537047	-	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
I3M_RS30610	5537112..5538218	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	5491419..5542086	50667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T100554 WP_000854753.1 NZ_CP028116:5533059-5533433 [Escherichia coli O26 str. RM8426]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T100554 NZ_CP028116:5533059-5533433 [Escherichia coli O26 str. RM8426]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT100554 WP_001315620.1 NZ_CP028116:5532601-5532969 [Escherichia coli O26 str. RM8426]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT100554 NZ_CP028116:5532601-5532969 [Escherichia coli O26 str. RM8426]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5