10055

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) darTG/-
Location 31528..34033 Replicon chromosome
Accession NZ_AJIP01000032
Organism Thermosipho africanus H17ap60334 Contig32

Toxin (Protein)


Gene name darT Uniprot ID K2PFJ6
Locus tag H17AP60334_RS06860 Protein ID WP_004103096.1
Coordinates 31528..32694 (+) Length 389 a.a.

Antitoxin (Protein)


Gene name darG Uniprot ID K2PBX9
Locus tag H17AP60334_RS06875 Protein ID WP_004103098.1
Coordinates 33497..34033 (+) Length 179 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H17AP60334_RS06845 (H17ap60334_07398) 27044..28447 + 1404 WP_004103090.1 dipeptidase PepV -
H17AP60334_RS06850 (H17ap60334_07403) 28527..29735 - 1209 WP_004103092.1 MFS transporter -
H17AP60334_RS06855 (H17ap60334_07408) 29929..30495 + 567 WP_004103094.1 DUF2202 domain-containing protein -
H17AP60334_RS06860 (H17ap60334_07413) 31528..32694 + 1167 WP_004103096.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein Toxin
H17AP60334_RS12490 32762..32998 + 237 WP_038042555.1 TIGR04255 family protein -
H17AP60334_RS06870 32995..33483 + 489 WP_038042557.1 TIGR04255 family protein -
H17AP60334_RS06875 (H17ap60334_07428) 33497..34033 + 537 WP_004103098.1 hypothetical protein Antitoxin
H17AP60334_RS06880 (H17ap60334_07433) 34041..34409 + 369 WP_004103100.1 HEPN domain-containing protein -
H17AP60334_RS11155 (H17ap60334_07438) 34716..34871 + 156 WP_004103102.1 DUF2202 domain-containing protein -
H17AP60334_RS06885 (H17ap60334_07443) 35530..36192 + 663 WP_198429593.1 hypothetical protein -
H17AP60334_RS06890 (H17ap60334_07448) 36580..37035 + 456 WP_004103107.1 hypothetical protein -
H17AP60334_RS06895 37227..37805 + 579 WP_232617955.1 sulfite exporter TauE/SafE family protein -
H17AP60334_RS06900 37958..38886 - 929 Protein_44 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Macro domain

(227-350)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 389 a.a.        Molecular weight: 44883.37 Da        Isoelectric Point: 9.4675

>T10055 WP_004103096.1 NZ_AJIP01000032:31528-32694 [Thermosipho africanus H17ap60334]
MFPRFRELYYITHIDNVPSILEKGILSHAEIERQSINCKKVYDNSIVLKRKSRLLADNRSLWEFANLYFQPRNPMLYRLL
VQGLKPKDLAIVAVKWTIMKRDDILITDGNAASSETQIYRKSEIKNIKNIISVKDMEYWREEDGSKRKIMAECLVPQCVD
PRYISAIYVSDHEVASNLKKAINNRNIPVIPDPTFFFLPNREIKLTQNLSLVEGDMFFSRMQTLTVSVNTVGVMGKGLAS
RVKYQFPDVYVVFQDACKKKELEFGKPYLYKRESSLDAFLAEDGEKLSDLNHQTWFLLFPTKRHWKNMSEIKGIESGLRW
IVENYKKEGIKSLAVPALGCGLGGLEWSIVGPLMCRYLTKLEIPVQIYLPLEKRIPDVQLSPKFLLDS

Download         Length: 1167 bp

>T10055 NZ_AJIP01000032:31528-32694 [Thermosipho africanus H17ap60334]
ATGTTCCCGAGATTTAGGGAACTTTATTATATAACACATATTGATAATGTGCCTTCTATTTTAGAAAAAGGAATTTTATC
CCACGCGGAAATTGAAAGGCAATCTATAAATTGCAAGAAGGTATACGACAATAGTATTGTATTAAAAAGAAAAAGTAGAT
TATTGGCTGATAACAGATCCCTTTGGGAATTTGCTAATTTGTACTTCCAACCAAGAAATCCTATGCTTTATCGCTTATTG
GTGCAAGGGCTAAAACCAAAAGATCTTGCGATAGTAGCTGTAAAATGGACAATAATGAAGAGGGATGATATATTAATTAC
TGATGGTAATGCTGCCTCGAGTGAGACGCAGATATACCGAAAAAGTGAGATAAAAAATATCAAAAATATTATTTCAGTTA
AGGATATGGAATATTGGCGCGAAGAAGATGGTTCGAAAAGAAAGATAATGGCTGAATGTTTGGTACCTCAGTGTGTTGAT
CCTAGATATATTAGTGCAATATATGTTAGTGACCACGAAGTAGCAAGCAATTTGAAAAAAGCAATAAACAACAGAAATAT
ACCTGTTATACCAGATCCTACTTTTTTCTTCTTACCAAATAGAGAGATTAAACTGACCCAGAATTTATCACTTGTTGAAG
GTGATATGTTCTTCTCAAGGATGCAAACATTAACAGTTAGTGTTAATACAGTTGGTGTAATGGGCAAAGGACTTGCTTCA
CGAGTGAAGTATCAATTTCCAGATGTTTATGTTGTTTTTCAAGACGCTTGCAAAAAGAAAGAACTTGAATTTGGTAAGCC
TTATTTGTACAAAAGGGAATCATCACTGGATGCATTTCTCGCTGAGGATGGCGAAAAGTTATCGGATCTCAATCACCAGA
CATGGTTTTTATTATTTCCTACTAAACGCCACTGGAAAAATATGTCAGAAATTAAAGGCATAGAATCTGGATTGCGTTGG
ATTGTTGAAAATTACAAAAAGGAAGGAATAAAATCCTTAGCGGTCCCTGCACTTGGTTGTGGACTTGGTGGTTTGGAATG
GTCAATTGTGGGGCCGTTAATGTGTAGATATTTGACTAAGCTTGAAATACCTGTACAAATTTATTTACCACTTGAAAAGC
GGATTCCGGATGTGCAATTATCACCGAAATTTTTGCTGGATAGTTGA

Antitoxin


Download         Length: 179 a.a.        Molecular weight: 21099.24 Da        Isoelectric Point: 7.0200

>AT10055 WP_004103098.1 NZ_AJIP01000032:33497-34033 [Thermosipho africanus H17ap60334]
MENNVIRTPLLENQLFNFPEEKSKTSEARKGIKIASRSLPFYDSSTYMQSFQRQIWPSSTKSTLTDKIYSFISNVIQMGV
HVEEDKKVSNYLLNFPDMQWVINIAIEVVKRYLPGVKLFLDLYQDPEIEDKFLVLYVRTTNYNRDFIEKLRKAEEEVIKL
LVDKQGWIQITSDFREVR

Download         Length: 537 bp

>AT10055 NZ_AJIP01000032:33497-34033 [Thermosipho africanus H17ap60334]
ATGGAGAATAATGTAATTAGAACACCACTTTTAGAAAACCAATTATTTAATTTTCCTGAGGAAAAATCTAAAACTTCTGA
AGCGAGAAAAGGAATTAAAATAGCGTCGAGGAGTTTACCTTTTTATGACTCATCTACTTACATGCAAAGCTTCCAGAGAC
AAATATGGCCTTCGAGTACAAAATCAACTTTAACTGATAAAATATACAGTTTTATAAGCAACGTTATTCAAATGGGAGTT
CATGTTGAGGAAGATAAAAAAGTTAGCAATTATCTTTTAAATTTTCCAGATATGCAATGGGTTATTAATATAGCAATTGA
AGTAGTTAAACGTTATTTACCAGGAGTAAAGTTATTTTTGGATCTCTATCAAGATCCGGAAATAGAAGATAAGTTTCTCG
TGTTGTATGTTAGAACTACAAATTATAATAGAGATTTCATAGAAAAGTTGAGGAAGGCAGAAGAAGAAGTTATAAAATTA
CTTGTTGATAAGCAAGGTTGGATACAGATTACTAGTGACTTTCGTGAAGTGAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7OMU

Antitoxin

Source ID Structure
AlphaFold DB K2PBX9

References

(1) Marion Schuller et al. (2021) Molecular basis for DarT ADP-ribosylation of a DNA base. Nature 596(7873):597-602. [PubMed:34408320] 3D_structure


3D_structure protein structure literature