Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3912110..3912764 Replicon chromosome
Accession NZ_CP028116
Organism Escherichia coli O26 str. RM8426

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I3M_RS22125 Protein ID WP_000244777.1
Coordinates 3912110..3912517 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I3M_RS22130 Protein ID WP_000354046.1
Coordinates 3912498..3912764 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3M_RS22105 3908067..3909800 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
I3M_RS22110 3909806..3910516 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I3M_RS22115 3910541..3911437 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I3M_RS22120 3911549..3912070 + 522 WP_001055874.1 flavodoxin FldB -
I3M_RS22125 3912110..3912517 - 408 WP_000244777.1 toxin CptA Toxin
I3M_RS22130 3912498..3912764 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I3M_RS22135 3913007..3913987 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
I3M_RS22140 3914183..3914842 - 660 WP_000250274.1 hemolysin III family protein -
I3M_RS22145 3915006..3915317 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
I3M_RS22150 3915362..3916795 + 1434 WP_000737106.1 6-phospho-beta-glucosidase BglA -
I3M_RS22155 3916852..3917595 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T100549 WP_000244777.1 NZ_CP028116:c3912517-3912110 [Escherichia coli O26 str. RM8426]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T100549 NZ_CP028116:c3912517-3912110 [Escherichia coli O26 str. RM8426]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT100549 WP_000354046.1 NZ_CP028116:c3912764-3912498 [Escherichia coli O26 str. RM8426]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT100549 NZ_CP028116:c3912764-3912498 [Escherichia coli O26 str. RM8426]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References