Detailed information of TA system TA100546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3412840..3413465
• Replicon: chromosome
• Accession: NZ_CP028116
• Organism: Escherichia coli O26 str. RM8426

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I3M_RS19560
• Protein ID: WP_000911330.1
• Coordinates: 3412840..3413238 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: I3M_RS19565
• Protein ID: WP_000450524.1
• Coordinates: 3413238..3413465 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3M_RS19540	3408718..3408918	+	201	WP_000383836.1	YpfN family protein	-
I3M_RS19545	3409028..3409726	-	699	WP_000679812.1	esterase	-
I3M_RS19550	3409800..3411815	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
I3M_RS19555	3411830..3412693	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
I3M_RS19560	3412840..3413238	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I3M_RS19565	3413238..3413465	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
I3M_RS19570	3413619..3414332	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
I3M_RS19575	3414545..3415579	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
I3M_RS19580	3415596..3416474	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
I3M_RS19585	3416620..3417192	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
I3M_RS19590	3417192..3417662	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T100546 WP_000911330.1 NZ_CP028116:c3413238-3412840 [Escherichia coli O26 str. RM8426]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T100546 NZ_CP028116:c3413238-3412840 [Escherichia coli O26 str. RM8426]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT100546 WP_000450524.1 NZ_CP028116:c3413465-3413238 [Escherichia coli O26 str. RM8426]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT100546 NZ_CP028116:c3413465-3413238 [Escherichia coli O26 str. RM8426]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33