Detailed information of TA system TA100532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1988417..1989055
• Replicon: chromosome
• Accession: NZ_CP028116
• Organism: Escherichia coli O26 str. RM8426

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: I3M_RS11735
• Protein ID: WP_000813794.1
• Coordinates: 1988417..1988593 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I3M_RS11740
• Protein ID: WP_001270286.1
• Coordinates: 1988639..1989055 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3M_RS11715	1984036..1985250	-	1215	WP_001307190.1	BenE family transporter YdcO	-
I3M_RS11720	1985303..1985839	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
I3M_RS11725	1985912..1987873	+	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I3M_RS11730	1987965..1988195	-	231	WP_000494244.1	YncJ family protein	-
I3M_RS11735	1988417..1988593	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I3M_RS11740	1988639..1989055	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I3M_RS11745	1989134..1990539	+	1406	Protein_2039	PLP-dependent aminotransferase family protein	-
I3M_RS11750	1990784..1991929	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
I3M_RS11755	1991947..1992960	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
I3M_RS11760	1992961..1993902	+	942	WP_001251332.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1982848..1983996	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T100532 WP_000813794.1 NZ_CP028116:1988417-1988593 [Escherichia coli O26 str. RM8426]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T100532 NZ_CP028116:1988417-1988593 [Escherichia coli O26 str. RM8426]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT100532 WP_001270286.1 NZ_CP028116:1988639-1989055 [Escherichia coli O26 str. RM8426]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT100532 NZ_CP028116:1988639-1989055 [Escherichia coli O26 str. RM8426]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB