Detailed information of TA system TA100524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1177151..1177629
• Replicon: chromosome
• Accession: NZ_CP028116
• Organism: Escherichia coli O26 str. RM8426

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: I3M_RS06625
• Protein ID: WP_001303876.1
• Coordinates: 1177151..1177438 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: I3M_RS06630
• Protein ID: WP_000536233.1
• Coordinates: 1177438..1177629 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3M_RS06590	1172374..1174824	-	2451	WP_106875240.1	exonuclease	-
I3M_RS06595	1174918..1175109	-	192	WP_001098307.1	DUF1482 family protein	-
I3M_RS06600	1175106..1175294	-	189	WP_000413705.1	cell division inhibition protein DicB	-
I3M_RS06610	1175862..1176071	+	210	WP_001133037.1	hypothetical protein	-
I3M_RS06615	1176072..1176710	-	639	WP_000394543.1	hypothetical protein	-
I3M_RS06620	1176722..1176874	-	153	WP_000380316.1	DUF1391 family protein	-
I3M_RS06625	1177151..1177438	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3M_RS06630	1177438..1177629	-	192	WP_000536233.1	hypothetical protein	Antitoxin
I3M_RS06635	1177657..1178058	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
I3M_RS06640	1178167..1178439	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
I3M_RS06645	1178423..1178848	+	426	WP_000693855.1	Rha family transcriptional regulator	-
I3M_RS06650	1179055..1179510	-	456	WP_000273724.1	hypothetical protein	-
I3M_RS06655	1179589..1180704	+	1116	WP_001205821.1	hypothetical protein	-
I3M_RS06660	1180711..1181457	+	747	WP_000788742.1	ATP-binding protein	-
I3M_RS06665	1181479..1182249	+	771	WP_000451007.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1163711..1184156	20445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T100524 WP_001303876.1 NZ_CP028116:c1177438-1177151 [Escherichia coli O26 str. RM8426]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T100524 NZ_CP028116:c1177438-1177151 [Escherichia coli O26 str. RM8426]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT100524 WP_000536233.1 NZ_CP028116:c1177629-1177438 [Escherichia coli O26 str. RM8426]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT100524 NZ_CP028116:c1177629-1177438 [Escherichia coli O26 str. RM8426]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA