Detailed information of TA system TA100520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9823..10466
• Replicon: plasmid pRM8426
• Accession: NZ_CP028115
• Organism: Escherichia coli O26 str. RM8426

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: I3M_RS00070
• Protein ID: WP_001044768.1
• Coordinates: 10050..10466 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: I3M_RS00065
• Protein ID: WP_001261287.1
• Coordinates: 9823..10053 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3M_RS00020	5688..7127	+	1440	WP_001213545.1	enterohemolysin T1SS ABC transporter subunit EhxD	-
I3M_RS00025	7194..7337	+	144	Protein_4	transcriptional regulator	-
I3M_RS00030	7341..7532	+	192	Protein_5	lipid II-degrading bacteriocin	-
I3M_RS00035	7540..7893	-	354	WP_000864810.1	colicin M immunity protein	-
I3M_RS00040	8066..8848	-	783	WP_001164205.1	site-specific integrase	-
I3M_RS00045	8850..9263	-	414	WP_000465041.1	hypothetical protein	-
I3M_RS00065	9823..10053	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I3M_RS00070	10050..10466	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
I3M_RS00075	10628..11173	-	546	WP_001165114.1	hypothetical protein	-
I3M_RS00080	11241..12397	+	1157	WP_085948186.1	IS3-like element IS600 family transposase	-
I3M_RS31690	12438..12587	-	150	WP_000955366.1	hypothetical protein	-
I3M_RS00085	13202..14062	-	861	WP_000704534.1	alpha/beta hydrolase	-
I3M_RS00090	14190..14576	+	387	WP_001066949.1	tyrosine-type recombinase/integrase	-
I3M_RS00095	14630..15304	+	675	WP_001341423.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB / katA / espP	1..90123	90123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T100520 WP_001044768.1 NZ_CP028115:10050-10466 [Escherichia coli O26 str. RM8426]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T100520 NZ_CP028115:10050-10466 [Escherichia coli O26 str. RM8426]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT100520 WP_001261287.1 NZ_CP028115:9823-10053 [Escherichia coli O26 str. RM8426]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT100520 NZ_CP028115:9823-10053 [Escherichia coli O26 str. RM8426]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4