Detailed information of TA system TA100519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 69142..69785
• Replicon: plasmid pRM8385-1
• Accession: NZ_CP028113
• Organism: Escherichia coli O103 str. RM8385

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: I3Q_RS30915
• Protein ID: WP_001044768.1
• Coordinates: 69142..69558 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: I3Q_RS30920
• Protein ID: WP_001261287.1
• Coordinates: 69555..69785 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Q_RS30890	65231..65578	-	348	WP_000631725.1	IS66 family insertion sequence element accessory protein TnpB	-
I3Q_RS30895	65575..66249	-	675	WP_001341423.1	IS66 family insertion sequence hypothetical protein	-
I3Q_RS30900	66303..66689	-	387	WP_001066949.1	tyrosine-type recombinase/integrase	-
I3Q_RS30905	66817..67677	+	861	WP_000704534.1	alpha/beta hydrolase	-
I3Q_RS30910	68435..68980	+	546	WP_001165114.1	hypothetical protein	-
I3Q_RS30915	69142..69558	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
I3Q_RS30920	69555..69785	-	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I3Q_RS30940	70345..70758	+	414	WP_000465041.1	hypothetical protein	-
I3Q_RS30945	70760..71542	+	783	WP_001164205.1	site-specific integrase	-
I3Q_RS30950	71715..72068	+	354	WP_000864810.1	colicin M immunity protein	-
I3Q_RS30955	72076..72267	-	192	Protein_75	lipid II-degrading bacteriocin	-
I3Q_RS30960	72271..72414	-	144	Protein_76	transcriptional regulator	-
I3Q_RS30965	72481..73920	-	1440	WP_001213545.1	enterohemolysin T1SS ABC transporter subunit EhxD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	espP / toxB / toxB / toxB / toxB / hlyD / hlyB / hlyA / hlyC	1..94220	94220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T100519 WP_001044768.1 NZ_CP028113:c69558-69142 [Escherichia coli O103 str. RM8385]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T100519 NZ_CP028113:c69558-69142 [Escherichia coli O103 str. RM8385]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT100519 WP_001261287.1 NZ_CP028113:c69785-69555 [Escherichia coli O103 str. RM8385]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT100519 NZ_CP028113:c69785-69555 [Escherichia coli O103 str. RM8385]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4