Detailed information of TA system TA100516

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5516783..5517615
• Replicon: chromosome
• Accession: NZ_CP028112
• Organism: Escherichia coli O103 str. RM8385

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: I3Q_RS29885
• Protein ID: WP_000854753.1
• Coordinates: 5517241..5517615 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: I3Q_RS29880
• Protein ID: WP_001315620.1
• Coordinates: 5516783..5517151 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Q_RS29845	5513619..5514074	+	456	WP_000581504.1	hypothetical protein	-
I3Q_RS29850	5514153..5514386	+	234	WP_001119729.1	DUF905 family protein	-
I3Q_RS29860	5514486..5515304	+	819	WP_001234622.1	DUF945 domain-containing protein	-
I3Q_RS29865	5515359..5515844	+	486	WP_000849588.1	antirestriction protein	-
I3Q_RS29870	5515860..5516336	+	477	WP_001186774.1	RadC family protein	-
I3Q_RS29875	5516399..5516620	+	222	WP_000692345.1	DUF987 domain-containing protein	-
I3Q_RS29880	5516783..5517151	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I3Q_RS29885	5517241..5517615	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
I3Q_RS29890	5517612..5518100	+	489	WP_000777547.1	hypothetical protein	-
I3Q_RS29895	5518112..5518309	+	198	WP_000839281.1	DUF957 domain-containing protein	-
I3Q_RS31805	5518394..5518546	+	153	WP_001280445.1	hypothetical protein	-
I3Q_RS29920	5519591..5520241	+	651	WP_001037966.1	HNH endonuclease	-
I3Q_RS29925	5520249..5521229	-	981	WP_106918322.1	sialate O-acetylesterase	-
I3Q_RS29930	5521294..5522400	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T100516 WP_000854753.1 NZ_CP028112:5517241-5517615 [Escherichia coli O103 str. RM8385]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T100516 NZ_CP028112:5517241-5517615 [Escherichia coli O103 str. RM8385]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT100516 WP_001315620.1 NZ_CP028112:5516783-5517151 [Escherichia coli O103 str. RM8385]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT100516 NZ_CP028112:5516783-5517151 [Escherichia coli O103 str. RM8385]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5