Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5256499..5256996 Replicon chromosome
Accession NZ_CP028112
Organism Escherichia coli O103 str. RM8385

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I3Q_RS28465 Protein ID WP_001173343.1
Coordinates 5256823..5256996 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I3Q_RS28460 Protein ID WP_000398619.1
Coordinates 5256499..5256795 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3Q_RS28435 5253230..5253949 - 720 WP_000611288.1 two-component system response regulator DcuR -
I3Q_RS28440 5253946..5255577 - 1632 WP_001216487.1 sensor histidine kinase -
I3Q_RS28445 5255758..5255988 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
I3Q_RS28450 5256000..5256272 + 273 WP_000405647.1 N-acetyltransferase -
I3Q_RS28460 5256499..5256795 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I3Q_RS28465 5256823..5256996 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I3Q_RS28470 5257115..5258632 - 1518 WP_001295074.1 lysine--tRNA ligase -
I3Q_RS28475 5258869..5260326 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T100514 WP_001173343.1 NZ_CP028112:5256823-5256996 [Escherichia coli O103 str. RM8385]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T100514 NZ_CP028112:5256823-5256996 [Escherichia coli O103 str. RM8385]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT100514 WP_000398619.1 NZ_CP028112:5256499-5256795 [Escherichia coli O103 str. RM8385]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT100514 NZ_CP028112:5256499-5256795 [Escherichia coli O103 str. RM8385]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References