Detailed information of TA system TA100513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 5122190..5123178
• Replicon: chromosome
• Accession: NZ_CP028112
• Organism: Escherichia coli O103 str. RM8385

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: I3Q_RS27655
• Protein ID: WP_014640052.1
• Coordinates: 5122792..5123178 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: A0A0J5Z6D5
• Locus tag: I3Q_RS27650
• Protein ID: WP_000458584.1
• Coordinates: 5122190..5122762 (+)
• Length: 191 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Q_RS27615	5117278..5117886	+	609	WP_000646078.1	transcriptional repressor LexA	-
I3Q_RS27620	5117959..5119284	+	1326	WP_001298861.1	MATE family efflux transporter DinF	-
I3Q_RS27625	5119400..5119609	+	210	WP_001030593.1	CsbD family protein	-
I3Q_RS27630	5119651..5120166	-	516	WP_001295691.1	zinc uptake transcriptional repressor Zur	-
I3Q_RS27640	5120484..5121469	+	986	Protein_5034	DUF2713 domain-containing protein	-
I3Q_RS27645	5121832..5121954	+	123	WP_001300034.1	hypothetical protein	-
I3Q_RS27650	5122190..5122762	+	573	WP_000458584.1	SocA family protein	Antitoxin
I3Q_RS27655	5122792..5123178	+	387	WP_014640052.1	hypothetical protein	Toxin
I3Q_RS31790	5123218..5123391	+	174	WP_000390073.1	hypothetical protein	-
I3Q_RS27660	5123439..5123720	-	282	WP_001093919.1	pyocin activator PrtN family protein	-
I3Q_RS27665	5123757..5124329	-	573	WP_001061338.1	3'-5' exoribonuclease	-
I3Q_RS27670	5124329..5124895	-	567	WP_000628776.1	DUF551 domain-containing protein	-
I3Q_RS31500	5125229..5125407	-	179	Protein_5042	hypothetical protein	-
I3Q_RS27680	5125409..5125957	-	549	WP_000192143.1	ead/Ea22-like family protein	-
I3Q_RS27685	5125954..5126178	-	225	WP_000015506.1	hypothetical protein	-
I3Q_RS27690	5126175..5126786	-	612	WP_001242710.1	hypothetical protein	-
I3Q_RS27695	5126777..5127313	-	537	WP_000008177.1	5'-deoxynucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stxA / stxB / nleG7'	5117278..5177322	60044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin SocA-like, Panacea domain (27-137)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14465.40 Da
• Isoelectric Point: 5.4833

>T100513 WP_014640052.1 NZ_CP028112:5122792-5123178 [Escherichia coli O103 str. RM8385]
VLAPTGPCNHLHVICNDPVYYPVNDCYCVLVVNISSIKDGVPHDPSCVLNSGDHRFIKHPSYVVYAEAIIWRVDNMVRKQ
RSGEISVHDDMPEATFNRILDGFDISDEVTPKNLKFKNKYCVSSIDDE

Nucleotide

• Length: 387 bp

>T100513 NZ_CP028112:5122792-5123178 [Escherichia coli O103 str. RM8385]
GTGCTTGCCCCAACTGGACCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCTGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATCTCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin

Protein

• Length: 191 a.a.
• Molecular weight: 21973.21 Da
• Isoelectric Point: 6.0283

>AT100513 WP_000458584.1 NZ_CP028112:5122190-5122762 [Escherichia coli O103 str. RM8385]
MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRQSILKHGRMIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYN
LIETNGHNVSLRSDPREMDADEVFDELSRADIRILDEIYSRYGHMNRFDLANMTHLESVCPEWHDPGNSRKPIDLKEMLI
SEGKSEDEANRIIGKMEESQKLKEFSLQLS

Nucleotide

• Length: 573 bp

>AT100513 NZ_CP028112:5122190-5122762 [Escherichia coli O103 str. RM8385]
ATGTTTTGCGAAGAAAAAGTAGCTCAAATGGCTGCATACCTACTTCTTAAGAGAGGTGGGCGCATGGCATATCTGAAATT
GATGAAGCTGCTCTATCTGTCTAACCGCCAGTCGATTTTGAAGCATGGCAGGATGATCGGCGAAGATAGCCTTTACTCTA
TGAAATTTGGACCAGTCATGTCGAATACGCTGAACTTGATTCGCGGTAAGGCTGAAGGCATTGGTGACTACTGGTACAAC
TTGATAGAGACGAACGGGCATAATGTATCGTTGCGTTCAGATCCGAGGGAAATGGATGCAGACGAGGTCTTTGATGAATT
GAGTCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCGGTATGGGCATATGAACCGATTTGATCTCGCAAATATGA
CGCATTTAGAAAGCGTTTGTCCAGAGTGGCACGATCCTGGCAATTCTCGTAAGCCTATAGACCTGAAAGAAATGCTGATC
AGTGAGGGTAAAAGCGAGGATGAGGCTAATCGCATAATTGGCAAAATGGAAGAATCTCAGAAACTTAAGGAATTTTCTTT
GCAATTATCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0J5Z6D5