Detailed information of TA system TA100505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2322776..2323254
• Replicon: chromosome
• Accession: NZ_CP028112
• Organism: Escherichia coli O103 str. RM8385

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: I3Q_RS12760
• Protein ID: WP_001303876.1
• Coordinates: 2322967..2323254 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: I3Q_RS12755
• Protein ID: WP_000536233.1
• Coordinates: 2322776..2322967 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Q_RS12720	2318181..2318951	-	771	WP_000451007.1	DUF1627 domain-containing protein	-
I3Q_RS12725	2318973..2319719	-	747	WP_000788750.1	ATP-binding protein	-
I3Q_RS12730	2319726..2320817	-	1092	WP_077756695.1	DNA-binding protein	-
I3Q_RS12735	2320896..2321351	+	456	WP_000273724.1	hypothetical protein	-
I3Q_RS12740	2321557..2321982	-	426	WP_000693855.1	Rha family transcriptional regulator	-
I3Q_RS12745	2321966..2322238	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
I3Q_RS12750	2322347..2322748	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
I3Q_RS12755	2322776..2322967	+	192	WP_000536233.1	hypothetical protein	Antitoxin
I3Q_RS12760	2322967..2323254	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3Q_RS12765	2323530..2323685	+	156	WP_000379575.1	DUF1391 family protein	-
I3Q_RS12775	2323827..2324216	+	390	WP_000394511.1	hypothetical protein	-
I3Q_RS12780	2324403..2324588	-	186	WP_001133046.1	hypothetical protein	-
I3Q_RS12790	2325162..2325350	+	189	WP_000413705.1	cell division inhibition protein DicB	-
I3Q_RS12795	2325347..2325538	+	192	WP_001098307.1	DUF1482 family protein	-
I3Q_RS12800	2325632..2328103	+	2472	WP_032317593.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espM2 / espW	2266409..2353832	87423

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T100505 WP_001303876.1 NZ_CP028112:2322967-2323254 [Escherichia coli O103 str. RM8385]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T100505 NZ_CP028112:2322967-2323254 [Escherichia coli O103 str. RM8385]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT100505 WP_000536233.1 NZ_CP028112:2322776-2322967 [Escherichia coli O103 str. RM8385]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT100505 NZ_CP028112:2322776-2322967 [Escherichia coli O103 str. RM8385]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA