Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 528771..529389 Replicon chromosome
Accession NZ_CP028112
Organism Escherichia coli O103 str. RM8385

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I3Q_RS02640 Protein ID WP_001291435.1
Coordinates 528771..528989 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I3Q_RS02645 Protein ID WP_000344800.1
Coordinates 529015..529389 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3Q_RS02605 524061..524633 + 573 WP_023442208.1 YbaY family lipoprotein -
I3Q_RS02610 524664..524975 - 312 WP_000409911.1 MGMT family protein -
I3Q_RS02620 525353..525706 + 354 WP_023442207.1 DUF1428 family protein -
I3Q_RS02625 525748..527298 - 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I3Q_RS02630 527462..527932 - 471 WP_000136192.1 YlaC family protein -
I3Q_RS02635 528048..528599 - 552 WP_000102564.1 maltose O-acetyltransferase -
I3Q_RS02640 528771..528989 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I3Q_RS02645 529015..529389 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I3Q_RS02650 529935..533084 - 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
I3Q_RS02655 533107..534300 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T100489 WP_001291435.1 NZ_CP028112:c528989-528771 [Escherichia coli O103 str. RM8385]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T100489 NZ_CP028112:c528989-528771 [Escherichia coli O103 str. RM8385]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT100489 WP_000344800.1 NZ_CP028112:c529389-529015 [Escherichia coli O103 str. RM8385]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT100489 NZ_CP028112:c529389-529015 [Escherichia coli O103 str. RM8385]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References