Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5053109..5053606 Replicon chromosome
Accession NZ_CP028110
Organism Escherichia coli O121 str. RM8352

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I3S_RS26865 Protein ID WP_001173343.1
Coordinates 5053433..5053606 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A3W5Y3H9
Locus tag I3S_RS26860 Protein ID WP_071532590.1
Coordinates 5053109..5053405 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3S_RS26835 5049840..5050559 - 720 WP_000611288.1 two-component system response regulator DcuR -
I3S_RS26840 5050556..5052187 - 1632 WP_001216468.1 sensor histidine kinase -
I3S_RS26845 5052368..5052598 + 231 WP_000371698.1 (4Fe-4S)-binding protein -
I3S_RS26850 5052610..5052882 + 273 WP_000405647.1 N-acetyltransferase -
I3S_RS26860 5053109..5053405 + 297 WP_071532590.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I3S_RS26865 5053433..5053606 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I3S_RS26870 5053725..5055242 - 1518 WP_001295082.1 lysine--tRNA ligase -
I3S_RS26875 5055479..5056936 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T100485 WP_001173343.1 NZ_CP028110:5053433-5053606 [Escherichia coli O121 str. RM8352]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T100485 NZ_CP028110:5053433-5053606 [Escherichia coli O121 str. RM8352]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11482.04 Da        Isoelectric Point: 4.2931

>AT100485 WP_071532590.1 NZ_CP028110:5053109-5053405 [Escherichia coli O121 str. RM8352]
MEGKKKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT100485 NZ_CP028110:5053109-5053405 [Escherichia coli O121 str. RM8352]
ATGGAAGGTAAAAAAAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A3W5Y3H9

References