Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3333372..3334032 Replicon chromosome
Accession NZ_CP028110
Organism Escherichia coli O121 str. RM8352

Toxin (Protein)


Gene name cptA Uniprot ID A0A3W5XVE6
Locus tag I3S_RS17770 Protein ID WP_000244778.1
Coordinates 3333619..3334032 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I3S_RS17765 Protein ID WP_000354046.1
Coordinates 3333372..3333638 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3S_RS17740 3328660..3329403 + 744 WP_000951948.1 SDR family oxidoreductase -
I3S_RS17745 3329460..3330893 - 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
I3S_RS17750 3330938..3331249 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
I3S_RS17755 3331413..3332072 + 660 WP_000250274.1 hemolysin III family protein -
I3S_RS17760 3332149..3333129 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
I3S_RS17765 3333372..3333638 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I3S_RS17770 3333619..3334032 + 414 WP_000244778.1 protein YgfX Toxin
I3S_RS17775 3334066..3334587 - 522 WP_001055874.1 flavodoxin FldB -
I3S_RS17780 3334699..3335595 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I3S_RS17785 3335619..3336329 + 711 WP_000715231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I3S_RS17790 3336335..3338068 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16275.23 Da        Isoelectric Point: 11.7064

>T100472 WP_000244778.1 NZ_CP028110:3333619-3334032 [Escherichia coli O121 str. RM8352]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

>T100472 NZ_CP028110:3333619-3334032 [Escherichia coli O121 str. RM8352]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT100472 WP_000354046.1 NZ_CP028110:3333372-3333638 [Escherichia coli O121 str. RM8352]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT100472 NZ_CP028110:3333372-3333638 [Escherichia coli O121 str. RM8352]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5XVE6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References