Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3244911..3245709 | Replicon | chromosome |
| Accession | NZ_CP028110 | ||
| Organism | Escherichia coli O121 str. RM8352 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A3W5Y7S6 |
| Locus tag | I3S_RS17260 | Protein ID | WP_000854916.1 |
| Coordinates | 3244911..3245288 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A3W5Y7S1 |
| Locus tag | I3S_RS17265 | Protein ID | WP_024174207.1 |
| Coordinates | 3245335..3245709 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I3S_RS17230 | 3240573..3241286 | - | 714 | Protein_3132 | DUF4942 domain-containing protein | - |
| I3S_RS17235 | 3241362..3242036 | + | 675 | WP_001341423.1 | IS66 family insertion sequence hypothetical protein | - |
| I3S_RS17240 | 3242033..3242380 | + | 348 | WP_000631725.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| I3S_RS17245 | 3242400..3243956 | + | 1557 | WP_001066419.1 | IS66 family transposase | - |
| I3S_RS29675 | 3243974..3244132 | - | 159 | WP_000014512.1 | hypothetical protein | - |
| I3S_RS17250 | 3244217..3244414 | - | 198 | WP_000839246.1 | DUF957 domain-containing protein | - |
| I3S_RS17255 | 3244426..3244914 | - | 489 | WP_000777666.1 | hypothetical protein | - |
| I3S_RS17260 | 3244911..3245288 | - | 378 | WP_000854916.1 | TA system toxin CbtA family protein | Toxin |
| I3S_RS17265 | 3245335..3245709 | - | 375 | WP_024174207.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| I3S_RS17270 | 3245872..3246093 | - | 222 | WP_000692312.1 | DUF987 domain-containing protein | - |
| I3S_RS17275 | 3246162..3246296 | - | 135 | Protein_3142 | hypothetical protein | - |
| I3S_RS17280 | 3246337..3247534 | + | 1198 | Protein_3143 | IS3 family transposase | - |
| I3S_RS17285 | 3247612..3247962 | - | 351 | WP_000624681.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| I3S_RS17290 | 3247959..3248384 | - | 426 | WP_000422707.1 | IS66 family insertion sequence hypothetical protein | - |
| I3S_RS17295 | 3248516..3248869 | - | 354 | Protein_3146 | transposase | - |
| I3S_RS29350 | 3248907..3249373 | + | 467 | Protein_3147 | transposase | - |
| I3S_RS29355 | 3249378..3249647 | - | 270 | Protein_3148 | transposase | - |
| I3S_RS17305 | 3249889..3250131 | + | 243 | WP_096855415.1 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | eae / escD / sepL / espA / espD / espB / cesD2 / escF / escG / espF / espK | 3224280..3259402 | 35122 | |
| - | inside | IScluster/Tn | - | - | 3239071..3254401 | 15330 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14033.04 Da Isoelectric Point: 9.1378
>T100471 WP_000854916.1 NZ_CP028110:c3245288-3244911 [Escherichia coli O121 str. RM8352]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIKAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITPGKHPEAKQ
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIKAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITPGKHPEAKQ
Download Length: 378 bp
>T100471 NZ_CP028110:c3245288-3244911 [Escherichia coli O121 str. RM8352]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTA
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCCGGGGAAGCATCCGGAGGCGAAACAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTA
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCCGGGGAAGCATCCGGAGGCGAAACAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13789.59 Da Isoelectric Point: 6.7387
>AT100471 WP_024174207.1 NZ_CP028110:c3245709-3245335 [Escherichia coli O121 str. RM8352]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTIYPTPATATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTIYPTPATATTS
Download Length: 375 bp
>AT100471 NZ_CP028110:c3245709-3245335 [Escherichia coli O121 str. RM8352]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTATTTATCCGACACCGGCAACCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTATTTATCCGACACCGGCAACCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3W5Y7S6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3W5Y7S1 |