Detailed information of TA system TA100470

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3193886..3194670
• Replicon: chromosome
• Accession: NZ_CP028110
• Organism: Escherichia coli O121 str. RM8352

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: C7BUQ8
• Locus tag: I3S_RS16945
• Protein ID: WP_000854717.1
• Coordinates: 3193886..3194212 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I3S_RS16950
• Protein ID: WP_001285487.1
• Coordinates: 3194302..3194670 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3S_RS16915	3189181..3190158	+	978	WP_000633206.1	type II secretion system minor pseudopilin GspK	-
I3S_RS16920	3190155..3191333	+	1179	WP_000094965.1	type II secretion system protein GspL	-
I3S_RS16925	3191335..3191871	+	537	WP_000942788.1	GspM family type II secretion system protein YghD	-
I3S_RS16930	3192214..3193056	-	843	WP_024174205.1	DUF4942 domain-containing protein	-
I3S_RS16935	3193141..3193338	-	198	WP_000839243.1	DUF957 domain-containing protein	-
I3S_RS16940	3193350..3193838	-	489	WP_000761730.1	hypothetical protein	-
I3S_RS16945	3193886..3194212	-	327	WP_000854717.1	TA system toxin CbtA family protein	Toxin
I3S_RS16950	3194302..3194670	-	369	WP_001285487.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I3S_RS16955	3194746..3194967	-	222	WP_000692312.1	DUF987 domain-containing protein	-
I3S_RS16960	3195030..3195506	-	477	WP_001186728.1	RadC family protein	-
I3S_RS16965	3195522..3195995	-	474	WP_000855068.1	antirestriction protein	-
I3S_RS16970	3196089..3196334	-	246	WP_001164974.1	hypothetical protein	-
I3S_RS16975	3196334..3196642	-	309	Protein_3082	DUF932 domain-containing protein	-
I3S_RS16985	3197924..3198130	-	207	WP_000632607.1	AlpA family transcriptional regulator	-
I3S_RS16990	3198620..3198793	+	174	Protein_3085	N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12194.91 Da
• Isoelectric Point: 6.4697

>T100470 WP_000854717.1 NZ_CP028110:c3194212-3193886 [Escherichia coli O121 str. RM8352]
MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDN

Nucleotide

• Length: 327 bp

>T100470 NZ_CP028110:c3194212-3193886 [Escherichia coli O121 str. RM8352]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.53 Da
• Isoelectric Point: 5.4844

>AT100470 WP_001285487.1 NZ_CP028110:c3194670-3194302 [Escherichia coli O121 str. RM8352]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT100470 NZ_CP028110:c3194670-3194302 [Escherichia coli O121 str. RM8352]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: C7BUQ8