Detailed information of TA system TA100469

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3070363..3071200
• Replicon: chromosome
• Accession: NZ_CP028110
• Organism: Escherichia coli O121 str. RM8352

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: I3S_RS16280
• Protein ID: WP_000854687.1
• Coordinates: 3070820..3071200 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LJJ3
• Locus tag: I3S_RS16275
• Protein ID: WP_001443392.1
• Coordinates: 3070363..3070731 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3S_RS16240	3065469..3065852	+	384	Protein_2948	DUF932 domain-containing protein	-
I3S_RS16245	3066362..3068140	+	1779	WP_001243192.1	reverse transcriptase/maturase family protein	-
I3S_RS16250	3068259..3068699	+	441	Protein_2950	DUF932 domain-containing protein	-
I3S_RS16255	3068699..3068944	+	246	WP_001164974.1	hypothetical protein	-
I3S_RS16260	3069038..3069511	+	474	WP_000855068.1	antirestriction protein	-
I3S_RS16265	3069527..3070003	+	477	WP_001186728.1	RadC family protein	-
I3S_RS16270	3070066..3070287	+	222	WP_000692312.1	DUF987 domain-containing protein	-
I3S_RS16275	3070363..3070731	+	369	WP_001443392.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I3S_RS16280	3070820..3071200	+	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
I3S_RS16285	3071197..3071685	+	489	WP_001054228.1	hypothetical protein	-
I3S_RS16290	3071705..3071902	+	198	WP_000772026.1	DUF957 domain-containing protein	-
I3S_RS16295	3071987..3072832	+	846	WP_001274557.1	DUF4942 domain-containing protein	-
I3S_RS16305	3073132..3073638	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
I3S_RS16310	3073717..3075558	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T100469 WP_000854687.1 NZ_CP028110:3070820-3071200 [Escherichia coli O121 str. RM8352]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T100469 NZ_CP028110:3070820-3071200 [Escherichia coli O121 str. RM8352]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13781.54 Da
• Isoelectric Point: 6.2049

>AT100469 WP_001443392.1 NZ_CP028110:3070363-3070731 [Escherichia coli O121 str. RM8352]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT100469 NZ_CP028110:3070363-3070731 [Escherichia coli O121 str. RM8352]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67

Antitoxin

• AlphaFold DB: A0A7U9LJJ3