Detailed information of TA system TA100464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 1956714..1957702
• Replicon: chromosome
• Accession: NZ_CP028110
• Organism: Escherichia coli O121 str. RM8352

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: I3S_RS10515
• Protein ID: WP_014640052.1
• Coordinates: 1956714..1957100 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: A0A0A1AFM1
• Locus tag: I3S_RS10520
• Protein ID: WP_000458583.1
• Coordinates: 1957130..1957702 (-)
• Length: 191 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3S_RS10475	1952873..1953208	+	336	WP_000543621.1	hypothetical protein	-
I3S_RS10480	1953199..1953549	+	351	WP_001242740.1	hypothetical protein	-
I3S_RS10485	1953546..1954019	+	474	WP_000145671.1	class I SAM-dependent methyltransferase	-
I3S_RS10490	1954166..1954633	+	468	WP_000829413.1	hypothetical protein	-
I3S_RS10495	1954635..1954826	+	192	WP_001014294.1	hypothetical protein	-
I3S_RS10500	1954829..1955563	+	735	WP_001094871.1	DUF551 domain-containing protein	-
I3S_RS10505	1955563..1956135	+	573	WP_001061339.1	3'-5' exoribonuclease	-
I3S_RS10510	1956172..1956453	+	282	WP_001093921.1	pyocin activator PrtN family protein	-
I3S_RS29630	1956501..1956674	-	174	WP_000390069.1	hypothetical protein	-
I3S_RS10515	1956714..1957100	-	387	WP_014640052.1	hypothetical protein	Toxin
I3S_RS10520	1957130..1957702	-	573	WP_000458583.1	SocA family protein	Antitoxin
I3S_RS10525	1957937..1958059	-	123	WP_001300034.1	hypothetical protein	-
I3S_RS10530	1958422..1959408	-	987	WP_000912572.1	DUF2713 domain-containing protein	-
I3S_RS10535	1959726..1960241	+	516	WP_001295691.1	zinc uptake transcriptional repressor Zur	-
I3S_RS10540	1960283..1960492	-	210	WP_001030593.1	CsbD family protein	-
I3S_RS10545	1960608..1961933	-	1326	WP_001370252.1	MATE family efflux transporter DinF	-
I3S_RS10550	1962006..1962614	-	609	WP_000646078.1	transcriptional repressor LexA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	1908596..1962614	54018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin SocA-like, Panacea domain (27-137)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14465.40 Da
• Isoelectric Point: 5.4833

>T100464 WP_014640052.1 NZ_CP028110:c1957100-1956714 [Escherichia coli O121 str. RM8352]
VLAPTGPCNHLHVICNDPVYYPVNDCYCVLVVNISSIKDGVPHDPSCVLNSGDHRFIKHPSYVVYAEAIIWRVDNMVRKQ
RSGEISVHDDMPEATFNRILDGFDISDEVTPKNLKFKNKYCVSSIDDE

Nucleotide

• Length: 387 bp

>T100464 NZ_CP028110:c1957100-1956714 [Escherichia coli O121 str. RM8352]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGGGTCCCCCACGATCCGTCTTGCGTCTTGAATTCCGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCTGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin

Protein

• Length: 191 a.a.
• Molecular weight: 21956.30 Da
• Isoelectric Point: 6.0283

>AT100464 WP_000458583.1 NZ_CP028110:c1957702-1957130 [Escherichia coli O121 str. RM8352]
MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRKSILKHGRMIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYN
LIETNGHDVLLRSDPREMDADEVFDELSRADIRILDEIYSLYGHMNRFDLANMTHLESVCPEWHNPGNSRKPIDLKEMLI
SEGKSEDEANRIIGKMEESQKLKEFSLQLS

Nucleotide

• Length: 573 bp

>AT100464 NZ_CP028110:c1957702-1957130 [Escherichia coli O121 str. RM8352]
ATGTTTTGCGAAGAAAAAGTAGCTCAAATGGCTGCATACCTACTTCTTAAGCGAGGTGGGCGCATGGCATATCTGAAATT
GATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGAAGATAGCCTTTACTCTA
TGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGCATTGGTGACTACTGGTACAAC
TTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGACGAGGTCTTTGATGAGTT
GAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATTTGATCTCGCAAACATGA
CGCATTTAGAAAGCGTTTGTCCAGAGTGGCACAATCCTGGCAATTCTCGTAAGCCTATAGACCTGAAAGAAATGCTGATC
AGTGAGGGTAAAAGCGAGGATGAGGCTAATCGCATAATTGGCAAAATGGAAGAATCTCAGAAACTTAAGGAATTTTCTTT
GCAATTATCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0A1AFM1