Detailed information of TA system TA100463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1839686..1840324
• Replicon: chromosome
• Accession: NZ_CP028110
• Organism: Escherichia coli O121 str. RM8352

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: I3S_RS09855
• Protein ID: WP_000813794.1
• Coordinates: 1840148..1840324 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I3S_RS09850
• Protein ID: WP_001270286.1
• Coordinates: 1839686..1840102 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3S_RS09830	1834838..1835779	-	942	WP_001251313.1	ABC transporter permease	-
I3S_RS09835	1835780..1836793	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
I3S_RS09840	1836811..1837956	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
I3S_RS09845	1838201..1839607	-	1407	WP_000760614.1	PLP-dependent aminotransferase family protein	-
I3S_RS09850	1839686..1840102	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I3S_RS09855	1840148..1840324	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I3S_RS09860	1840546..1840776	+	231	WP_000494244.1	YncJ family protein	-
I3S_RS09865	1840868..1842829	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I3S_RS09870	1842902..1843438	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
I3S_RS09875	1843491..1844705	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T100463 WP_000813794.1 NZ_CP028110:c1840324-1840148 [Escherichia coli O121 str. RM8352]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T100463 NZ_CP028110:c1840324-1840148 [Escherichia coli O121 str. RM8352]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT100463 WP_001270286.1 NZ_CP028110:c1840102-1839686 [Escherichia coli O121 str. RM8352]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT100463 NZ_CP028110:c1840102-1839686 [Escherichia coli O121 str. RM8352]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCACCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB