Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1290764..1291235 Replicon chromosome
Accession NZ_CP028110
Organism Escherichia coli O121 str. RM8352

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag I3S_RS06650 Protein ID WP_001303511.1
Coordinates 1290764..1291042 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag I3S_RS06655 Protein ID WP_001302048.1
Coordinates 1291044..1291235 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3S_RS06620 1286252..1286521 - 270 WP_000003742.1 excisionase -
I3S_RS06625 1286583..1289054 - 2472 WP_000048560.1 exonuclease -
I3S_RS06630 1289178..1289399 - 222 WP_000560212.1 cell division protein FtsZ -
I3S_RS06640 1290112..1290411 - 300 WP_001240334.1 hypothetical protein -
I3S_RS06650 1290764..1291042 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I3S_RS06655 1291044..1291235 - 192 WP_001302048.1 hypothetical protein Antitoxin
I3S_RS06660 1291256..1291627 - 372 WP_001169687.1 hypothetical protein -
I3S_RS06665 1291725..1292027 + 303 WP_000172738.1 transcriptional regulator -
I3S_RS06670 1292024..1292449 + 426 WP_000693944.1 Rha family transcriptional regulator -
I3S_RS06675 1292472..1293434 + 963 WP_000095671.1 helix-turn-helix domain-containing protein -
I3S_RS06680 1293475..1293897 + 423 WP_001151187.1 DUF977 family protein -
I3S_RS06685 1294003..1294215 + 213 WP_000935423.1 hypothetical protein -
I3S_RS06690 1294248..1294466 + 219 WP_000209148.1 DUF4014 family protein -
I3S_RS29195 1294468..1294626 + 159 WP_001370098.1 hypothetical protein -
I3S_RS06695 1294630..1296186 - 1557 WP_001066422.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1281317..1331622 50305


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T100458 WP_001303511.1 NZ_CP028110:c1291042-1290764 [Escherichia coli O121 str. RM8352]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T100458 NZ_CP028110:c1291042-1290764 [Escherichia coli O121 str. RM8352]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT100458 WP_001302048.1 NZ_CP028110:c1291235-1291044 [Escherichia coli O121 str. RM8352]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT100458 NZ_CP028110:c1291235-1291044 [Escherichia coli O121 str. RM8352]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References