Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 494456..495074 Replicon chromosome
Accession NZ_CP028110
Organism Escherichia coli O121 str. RM8352

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I3S_RS02405 Protein ID WP_001291435.1
Coordinates 494456..494674 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I3S_RS02410 Protein ID WP_000344800.1
Coordinates 494700..495074 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3S_RS02370 489746..490318 + 573 WP_000779826.1 lipoprotein -
I3S_RS02375 490349..490660 - 312 WP_000409911.1 MGMT family protein -
I3S_RS02385 491039..491392 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I3S_RS02390 491434..492984 - 1551 WP_001370355.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I3S_RS02395 493148..493618 - 471 WP_000136192.1 YlaC family protein -
I3S_RS02400 493734..494285 - 552 WP_000102569.1 maltose O-acetyltransferase -
I3S_RS02405 494456..494674 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I3S_RS02410 494700..495074 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I3S_RS02415 495620..498769 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I3S_RS02420 498792..499985 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T100456 WP_001291435.1 NZ_CP028110:c494674-494456 [Escherichia coli O121 str. RM8352]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T100456 NZ_CP028110:c494674-494456 [Escherichia coli O121 str. RM8352]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT100456 WP_000344800.1 NZ_CP028110:c495074-494700 [Escherichia coli O121 str. RM8352]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT100456 NZ_CP028110:c495074-494700 [Escherichia coli O121 str. RM8352]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References