Detailed information of TA system TA100451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 92097..92590
• Replicon: chromosome
• Accession: NZ_CP028107
• Organism: Fusobacterium necrophorum subsp. funduliforme strain 1_1_36S

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4N15_RS00490
• Protein ID: WP_005955952.1
• Coordinates: 92324..92590 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J5W0H3
• Locus tag: C4N15_RS00485
• Protein ID: WP_005955951.1
• Coordinates: 92097..92327 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4N15_RS00460	87338..87649	-	312	WP_005955931.1	50S ribosomal protein L21	-
C4N15_RS00465	87774..89441	-	1668	WP_106894724.1	tetratricopeptide repeat protein	-
C4N15_RS00470	89484..89960	-	477	WP_005955939.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
C4N15_RS00475	89976..90950	-	975	WP_005956947.1	D-glycero-beta-D-manno-heptose-7-phosphate kinase	-
C4N15_RS00480	91076..91594	-	519	WP_005957007.1	cob(I)yrinic acid a,c-diamide adenosyltransferase	-
C4N15_RS00485	92097..92327	+	231	WP_005955951.1	hypothetical protein	Antitoxin
C4N15_RS00490	92324..92590	+	267	WP_005955952.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4N15_RS00500	93316..94617	+	1302	WP_035935070.1	MATE family efflux transporter	-
C4N15_RS00505	94649..95398	+	750	WP_106894725.1	YebC/PmpR family DNA-binding transcriptional regulator	-
C4N15_RS00510	95440..97482	+	2043	WP_005955964.1	ATP-dependent DNA helicase RecG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-87)

Antitoxin

• Protein of unknown function DUF6290 (1-72)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10633.55 Da
• Isoelectric Point: 10.2235

>T100451 WP_005955952.1 NZ_CP028107:92324-92590 [Fusobacterium necrophorum subsp. funduliforme]
MKYRMEFSQQGKKELKKLDAFTQKIIMKWICKNLIDTENPRIHGKELKGNLKCFWRYRVGNYRLLADIQDETVTILSIKI
GHRKEIYE

Nucleotide

• Length: 267 bp

>T100451 NZ_CP028107:92324-92590 [Fusobacterium necrophorum subsp. funduliforme]
ATGAAATACCGAATGGAATTTTCACAACAAGGAAAAAAGGAATTGAAAAAATTAGATGCCTTTACTCAAAAAATTATTAT
GAAGTGGATCTGTAAAAATCTAATTGATACGGAAAATCCAAGAATACATGGAAAAGAACTAAAGGGAAACTTAAAGTGCT
TTTGGCGTTATCGTGTCGGAAATTACAGGTTATTAGCCGATATTCAAGACGAAACGGTAACCATTTTATCAATCAAAATA
GGACACAGAAAAGAAATTTATGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9012.25 Da
• Isoelectric Point: 4.3363

>AT100451 WP_005955951.1 NZ_CP028107:92097-92327 [Fusobacterium necrophorum subsp. funduliforme]
MSIISLRLNEKEEKLLREFSAFEGLGISSYIKKIIYERLEDEYDIKCFDKAYQEYLESGKKSYSFDEVLNELGIEL

Nucleotide

• Length: 231 bp

>AT100451 NZ_CP028107:92097-92327 [Fusobacterium necrophorum subsp. funduliforme]
ATGTCTATTATTTCTTTACGACTAAATGAAAAAGAAGAAAAATTATTAAGAGAATTCTCAGCATTTGAGGGCTTGGGAAT
CTCCTCATATATCAAAAAAATTATCTATGAAAGATTAGAGGATGAGTATGATATTAAATGTTTTGATAAAGCATATCAGG
AATATCTGGAAAGTGGAAAAAAATCATATTCTTTTGATGAAGTTTTAAATGAATTGGGAATTGAGCTATGA

Structures

Antitoxin

• AlphaFold DB: J5W0H3