Detailed information of TA system TA100445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /PIN(toxin)
• Location: 9713..10373
• Replicon: plasmid pSYSG
• Accession: NZ_CP028098
• Organism: Synechocystis sp. IPPAS B-1465

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: C7I86_RS17285
• Protein ID: WP_011153785.1
• Coordinates: 9948..10373 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: C7I86_RS17280
• Protein ID: WP_011153784.1
• Coordinates: 9713..9940 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7I86_RS17255	6043..6393	-	351	WP_011153778.1	DUF5615 family PIN-like protein	-
C7I86_RS17260	6377..6601	-	225	WP_011153779.1	DUF433 domain-containing protein	-
C7I86_RS17265	6652..8619	-	1968	Protein_9	SAM-dependent DNA methyltransferase	-
C7I86_RS17270	8821..9195	-	375	WP_011153782.1	DUF5615 family PIN-like protein	-
C7I86_RS17275	9205..9621	-	417	WP_011153783.1	hypothetical protein	-
C7I86_RS17280	9713..9940	+	228	WP_011153784.1	DUF2281 domain-containing protein	Antitoxin
C7I86_RS17285	9948..10373	+	426	WP_011153785.1	PIN domain-containing protein	Toxin
C7I86_RS17290	10395..10994	+	600	WP_011153786.1	ParA family protein	-
C7I86_RS17295	10998..11867	+	870	WP_011153787.1	ParB/RepB/Spo0J family partition protein	-
C7I86_RS17305	12618..13151	-	534	WP_011153788.1	hypothetical protein	-
C7I86_RS17310	13695..15287	-	1593	WP_011153789.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..44343	44343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-116)

Antitoxin

• Domain of unknown function DUF2281 (6-66)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15962.62 Da
• Isoelectric Point: 4.3429

>T100445 WP_011153785.1 NZ_CP028098:9948-10373 [Synechocystis sp. IPPAS B-1465]
VKVLLDTNIVLDLLLEREPFIEETIAIFEEIEQGNIEAYIAATTVTNIFYIIRKNKSREVALIAVKRLLIGLQLCPVNLQ
VIEVALDINLRDFEDSVQLACATVNQLTAIITRDTKDFVESPLPIYSPAECLYRVKTNKLD

Nucleotide

• Length: 426 bp

>T100445 NZ_CP028098:9948-10373 [Synechocystis sp. IPPAS B-1465]
GTGAAAGTACTTCTCGACACCAATATTGTTCTCGACTTACTTTTAGAGAGAGAACCGTTTATCGAAGAAACCATAGCCAT
CTTTGAAGAAATCGAGCAGGGAAACATCGAAGCTTACATTGCGGCTACAACAGTTACCAATATTTTTTACATCATTCGTA
AGAACAAAAGCCGCGAGGTGGCGCTTATAGCTGTTAAGCGACTACTGATTGGACTACAACTATGCCCCGTGAATCTACAA
GTTATCGAAGTTGCCCTCGACATCAATCTAAGAGACTTTGAAGATAGTGTCCAATTGGCATGTGCCACAGTTAACCAACT
TACTGCAATCATTACCCGTGACACCAAAGACTTTGTTGAGAGTCCTTTGCCTATTTACTCCCCTGCGGAATGTTTATATC
GGGTTAAGACCAATAAGCTTGATTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8420.71 Da
• Isoelectric Point: 6.2748

>AT100445 WP_011153784.1 NZ_CP028098:9713-9940 [Synechocystis sp. IPPAS B-1465]
MTAQEKLYQLIQTLPEKEVSEILNFAESLWPKVSSPKIPAGTLTGLRGIAKPTGPVPSDAELQRQYTDYLKEKYL

Nucleotide

• Length: 228 bp

>AT100445 NZ_CP028098:9713-9940 [Synechocystis sp. IPPAS B-1465]
ATGACAGCGCAAGAAAAACTCTACCAACTAATTCAAACTCTGCCAGAGAAGGAAGTTAGCGAAATACTCAATTTTGCCGA
GTCGCTTTGGCCAAAAGTCTCTAGCCCCAAAATTCCCGCCGGCACCCTTACCGGACTGCGGGGCATTGCCAAACCAACGG
GCCCCGTACCTAGCGACGCTGAGTTACAAAGGCAATACACTGACTACCTAAAAGAGAAGTATCTGTAA