Detailed information of TA system TA100441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2516990..2517589
• Replicon: chromosome
• Accession: NZ_CP028094
• Organism: Synechocystis sp. IPPAS B-1465

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C7I86_RS11810
• Protein ID: WP_010873447.1
• Coordinates: 2516990..2517367 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C7I86_RS11815
• Protein ID: WP_010873448.1
• Coordinates: 2517368..2517589 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7I86_RS11795	2512354..2513049	-	696	WP_010873444.1	aldehyde oxygenase (deformylating)	-
C7I86_RS11800	2513307..2514404	-	1098	WP_014407137.1	glucose-1-phosphate thymidylyltransferase	-
C7I86_RS11805	2514646..2516814	+	2169	WP_010873446.1	glycine--tRNA ligase subunit beta	-
C7I86_RS11810	2516990..2517367	-	378	WP_010873447.1	type II toxin-antitoxin system VapC family toxin	Toxin
C7I86_RS11815	2517368..2517589	-	222	WP_010873448.1	DUF2281 domain-containing protein	Antitoxin
C7I86_RS11820	2517654..2519024	-	1371	WP_010873449.1	FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (2-124)

Antitoxin

• Domain of unknown function DUF2281 (6-72)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14761.18 Da
• Isoelectric Point: 7.0607

>T100441 WP_010873447.1 NZ_CP028094:c2517367-2516990 [Synechocystis sp. IPPAS B-1465]
MLLDTHTLLWFLDNDIKLPSHLKSLIEAKPKVYVSIVSLWEIAIKLNINKLRLNYAFTDLENLLADLNISVLNITWQHLE
ACRNLPLHHRDPFDRMIIAQAQQHNFSVISKDENFKKYNVNLIWE

Nucleotide

• Length: 378 bp

>T100441 NZ_CP028094:c2517367-2516990 [Synechocystis sp. IPPAS B-1465]
ATGCTACTGGACACCCACACGCTTTTGTGGTTTTTAGATAATGATATAAAACTACCATCACATCTCAAGAGTTTGATTGA
AGCCAAGCCAAAAGTTTATGTCAGCATTGTATCTCTCTGGGAGATTGCCATTAAACTCAACATCAACAAACTAAGGCTTA
ATTACGCTTTTACTGATCTAGAGAATTTACTTGCTGATCTCAATATTTCGGTTTTGAACATCACATGGCAACATTTGGAA
GCTTGTCGCAATTTGCCCTTGCATCATCGAGATCCTTTTGACCGTATGATTATTGCCCAGGCTCAGCAACATAATTTTTC
CGTCATTAGCAAAGACGAAAATTTCAAAAAATACAACGTTAATTTAATCTGGGAATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8355.66 Da
• Isoelectric Point: 4.3855

>AT100441 WP_010873448.1 NZ_CP028094:c2517589-2517368 [Synechocystis sp. IPPAS B-1465]
MTDTALLEKINALPDAMKIEVEHFVEFLLTKQPPVSPVTNDGQKKYRQAGVLKGKIWMADDFDAPLEEMQEYM

Nucleotide

• Length: 222 bp

>AT100441 NZ_CP028094:c2517589-2517368 [Synechocystis sp. IPPAS B-1465]
ATGACAGACACCGCCCTACTAGAAAAGATCAATGCTCTCCCCGATGCCATGAAAATTGAAGTAGAACACTTTGTCGAATT
TCTGCTGACTAAGCAACCACCAGTCAGTCCAGTAACCAATGATGGACAAAAAAAATATCGTCAAGCAGGTGTTCTAAAGG
GGAAAATTTGGATGGCAGATGACTTTGATGCTCCTTTGGAAGAAATGCAGGAGTATATGTAA