Detailed information of TA system TA100436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 473953..474432
• Replicon: chromosome
• Accession: NZ_CP028094
• Organism: Synechocystis sp. IPPAS B-1465

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C7I86_RS02270
• Protein ID: WP_010871640.1
• Coordinates: 473953..474204 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C7I86_RS02275
• Protein ID: WP_010871641.1
• Coordinates: 474205..474432 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7I86_RS02250	469001..469546	-	546	WP_010871636.1	alpha/beta hydrolase	-
C7I86_RS02255	469592..470914	+	1323	WP_010871637.1	hypothetical protein	-
C7I86_RS02260	471080..472930	+	1851	WP_010871638.1	ATP-dependent zinc metalloprotease FtsH3	-
C7I86_RS02265	473088..473675	+	588	Protein_445	IS5 family transposase	-
C7I86_RS02270	473953..474204	-	252	WP_010871640.1	type II toxin-antitoxin system VapC family toxin	Toxin
C7I86_RS02275	474205..474432	-	228	WP_010871641.1	virulence factor	Antitoxin
C7I86_RS02280	474511..474792	-	282	WP_020861443.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
C7I86_RS02285	474789..475007	-	219	WP_010871642.1	hypothetical protein	-
C7I86_RS02290	475110..475445	-	336	WP_010871643.1	DUF86 domain-containing protein	-
C7I86_RS02295	475435..475743	-	309	WP_010871644.1	nucleotidyltransferase family protein	-
C7I86_RS02300	475873..477399	-	1527	WP_010871645.1	RNA-directed DNA polymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	473414..473662	248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9540.13 Da
• Isoelectric Point: 7.9952

>T100436 WP_010871640.1 NZ_CP028094:c474204-473953 [Synechocystis sp. IPPAS B-1465]
MKYLLDTNICIYLIKKKPFKVLAKFQTLEISDIGISSITVAELEYGVSKSQQQSKNRDALMQFLMPLEIVEFNSGSGDRL
WQH

Nucleotide

• Length: 252 bp

>T100436 NZ_CP028094:c474204-473953 [Synechocystis sp. IPPAS B-1465]
ATGAAGTATTTACTAGATACGAATATTTGCATTTATCTCATTAAGAAGAAACCGTTTAAAGTCTTGGCAAAATTTCAAAC
CCTGGAGATATCAGACATTGGTATTTCATCCATTACCGTGGCGGAATTAGAATATGGTGTCAGTAAAAGTCAACAACAAT
CGAAAAATCGTGATGCCTTAATGCAATTCTTGATGCCGCTAGAAATCGTGGAATTTAATTCAGGAAGCGGCGATCGCCTA
TGGCAACATTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8538.69 Da
• Isoelectric Point: 4.2840

>AT100436 WP_010871641.1 NZ_CP028094:c474432-474205 [Synechocystis sp. IPPAS B-1465]
MNTAQISTDGTHQIVILPENFTIAGSEVYIKKIGSTIILIAKNNPWQSLIESLDQFSDDFMKTREQPPLDIREEF

Nucleotide

• Length: 228 bp

>AT100436 NZ_CP028094:c474432-474205 [Synechocystis sp. IPPAS B-1465]
ATGAACACAGCTCAAATTAGCACCGATGGCACCCATCAAATCGTTATTTTACCAGAAAACTTTACGATAGCCGGAAGCGA
AGTTTACATTAAAAAAATTGGAAGTACAATTATTTTGATTGCTAAAAATAACCCTTGGCAATCCTTAATTGAAAGCTTAG
ATCAATTTTCAGATGATTTTATGAAAACTAGGGAACAGCCTCCCTTAGATATACGAGAAGAGTTCTAA