Detailed information of TA system TA100435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 3748803..3749439
• Replicon: chromosome
• Accession: NZ_CP028083
• Organism: Lysinibacillus sp. SGAir0095

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C1N55_RS18405
• Protein ID: WP_107937700.1
• Coordinates: 3748803..3749153 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: C1N55_RS18410
• Protein ID: WP_137730139.1
• Coordinates: 3749158..3749439 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1N55_RS18380	3745459..3746238	-	780	WP_137730134.1	RNA polymerase sigma factor SigB	-
C1N55_RS18385	3746216..3746689	-	474	WP_137730135.1	anti-sigma B factor RsbW	-
C1N55_RS18390	3746676..3747011	-	336	WP_137730136.1	STAS domain-containing protein	-
C1N55_RS18395	3747087..3748085	-	999	WP_137730137.1	PP2C family protein-serine/threonine phosphatase	-
C1N55_RS18400	3748099..3748497	-	399	WP_137730138.1	anti-sigma regulatory factor	-
C1N55_RS18405	3748803..3749153	-	351	WP_107937700.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C1N55_RS18410	3749158..3749439	-	282	WP_137730139.1	transcriptional regulator	Antitoxin
C1N55_RS18415	3749583..3750704	-	1122	WP_137730140.1	alanine racemase	-
C1N55_RS18420	3750920..3751933	-	1014	WP_137730141.1	outer membrane lipoprotein carrier protein LolA	-
C1N55_RS18425	3752064..3752420	-	357	WP_137730142.1	holo-ACP synthase	-
C1N55_RS18430	3752727..3753332	+	606	WP_137730143.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12953.10 Da
• Isoelectric Point: 5.8752

>T100435 WP_107937700.1 NZ_CP028083:c3749153-3748803 [Lysinibacillus sp. SGAir0095]
MIVKRGDVFFADLSPVVGSEQGGTRPVLILQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKKYGFERNSVILLEQL
RTIDKSRLTDRITQLDDELMEKVDVALEISLGLMKF

Nucleotide

• Length: 351 bp

>T100435 NZ_CP028083:c3749153-3748803 [Lysinibacillus sp. SGAir0095]
TTGATTGTGAAACGAGGGGACGTGTTTTTTGCAGATCTCTCACCAGTTGTGGGATCTGAACAAGGCGGTACGAGGCCCGT
ATTAATCCTTCAAAACGATATAGGTAATCGGTTTAGTCCAACTGTAATCATCGCTGCGATTACTGCGCAAATTCAAAAAG
CAAAATTGCCGACTCATGTCGAAATTGATGCTAAAAAGTATGGTTTCGAAAGAAATTCGGTAATTTTGCTTGAACAATTG
CGTACGATAGATAAATCTAGGTTGACAGATCGTATAACCCAATTAGATGATGAACTTATGGAAAAAGTGGACGTTGCACT
TGAAATAAGTCTAGGATTAATGAAATTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10825.41 Da
• Isoelectric Point: 5.1923

>AT100435 WP_137730139.1 NZ_CP028083:c3749439-3749158 [Lysinibacillus sp. SGAir0095]
MYAKKVEEVKEVLELPQEMLMAFNTLEQKSRESESFVYISSRRLSTHKQPNQIREALMKGYVEMSQINLNICSECLHAEY
EAEHMVERLVSGG

Nucleotide

• Length: 282 bp

>AT100435 NZ_CP028083:c3749439-3749158 [Lysinibacillus sp. SGAir0095]
TTGTACGCGAAAAAAGTAGAAGAAGTAAAGGAAGTATTAGAACTTCCTCAAGAAATGTTAATGGCATTTAATACACTTGA
GCAAAAATCACGAGAAAGTGAAAGTTTTGTTTATATTTCATCTCGTAGATTGTCTACGCACAAGCAACCAAATCAAATAC
GGGAAGCTTTAATGAAAGGCTATGTGGAAATGTCGCAAATCAACCTGAACATTTGTAGCGAATGTCTACATGCAGAGTAT
GAAGCGGAACATATGGTAGAGCGTCTCGTAAGCGGGGGATGA