Detailed information of TA system TA100424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1729930..1730727
• Replicon: chromosome
• Accession: NZ_CP027986
• Organism: Enterobacter sichuanensis strain SGAir0282

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: C1N69_RS08180
• Protein ID: WP_155030973.1
• Coordinates: 1730206..1730727 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0F1BC27
• Locus tag: C1N69_RS08175
• Protein ID: WP_025755885.1
• Coordinates: 1729930..1730199 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1N69_RS08145	1725557..1726000	-	444	WP_094918320.1	helix-turn-helix transcriptional regulator	-
C1N69_RS08150	1726153..1727394	+	1242	WP_059385533.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
C1N69_RS08155	1727427..1727654	-	228	WP_008499832.1	YccJ family protein	-
C1N69_RS08160	1727673..1728269	-	597	WP_025755888.1	NAD(P)H:quinone oxidoreductase	-
C1N69_RS08165	1728662..1728832	+	171	WP_013097201.1	general stress protein	-
C1N69_RS08170	1728951..1729868	+	918	WP_095451688.1	DMT family transporter	-
C1N69_RS08175	1729930..1730199	+	270	WP_025755885.1	DUF1778 domain-containing protein	Antitoxin
C1N69_RS08180	1730206..1730727	+	522	WP_155030973.1	GNAT family N-acetyltransferase	Toxin
C1N69_RS08185	1730802..1732124	-	1323	WP_155030974.1	pyrimidine utilization transport protein G	-
C1N69_RS08190	1732146..1732640	-	495	WP_095451691.1	pyrimidine utilization flavin reductase protein F	-
C1N69_RS08195	1732650..1733240	-	591	WP_094918323.1	malonic semialdehyde reductase	-
C1N69_RS08200	1733250..1734050	-	801	WP_155030975.1	pyrimidine utilization protein D	-
C1N69_RS08205	1734058..1734444	-	387	WP_047958241.1	pyrimidine utilization protein C	-
C1N69_RS08210	1734456..1735145	-	690	WP_155030976.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19818.62 Da
• Isoelectric Point: 7.1163

>T100424 WP_155030973.1 NZ_CP027986:1730206-1730727 [Enterobacter sichuanensis]
VENLTIEMFSEGEEYDFRNFDCGEPSLNSFLADHLVRQHTGRILRGYLLKDRDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYINVPSVTLGRLAVHKDLQGREWGTTLVTHAMRVVYLASQAVGVHGLFVDAINEQAKRFYLKLGFIPLTGENSHSLF
YPTKSIERLLEEE

Nucleotide

• Length: 522 bp

>T100424 NZ_CP027986:1730206-1730727 [Enterobacter sichuanensis]
GTGGAAAATCTCACGATCGAAATGTTTTCAGAGGGAGAAGAGTATGATTTCAGGAATTTCGATTGTGGTGAACCCTCGCT
AAATTCCTTCCTGGCAGACCATCTGGTGCGTCAGCACACTGGGCGTATTTTACGCGGCTATCTGCTTAAAGATCGCGACC
ATCCTCGTGTCCTCGGCTATTACACGCTGTCCGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATATCAACGTACCGAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGACCTGCAGGGACGTGAGTGGGG
AACAACACTCGTCACGCATGCTATGCGCGTGGTTTACCTGGCATCGCAGGCGGTAGGCGTACATGGACTCTTTGTCGATG
CGATTAACGAACAGGCTAAACGGTTTTATCTGAAGCTGGGCTTTATCCCTCTGACGGGTGAAAACAGTCATTCGCTTTTT
TATCCCACCAAATCTATCGAACGCCTGCTTGAAGAAGAGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10291.76 Da
• Isoelectric Point: 7.0174

>AT100424 WP_025755885.1 NZ_CP027986:1729930-1730199 [Enterobacter sichuanensis]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNETSWNAVMNAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT100424 NZ_CP027986:1729930-1730199 [Enterobacter sichuanensis]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCCTGACGGACGAAGACAAAACAATGATTGAAGAGGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCTGAACGTGCTGCAGAGGTCATCGAACAGCATCGGCGTC
TCGTCTTAAACGAAACATCATGGAATGCCGTGATGAACGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0F1BC27