Detailed information of TA system TA100422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 509713..510229
• Replicon: chromosome
• Accession: NZ_CP027986
• Organism: Enterobacter sichuanensis strain SGAir0282

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C1N69_RS02445
• Protein ID: WP_025759089.1
• Coordinates: 509945..510229 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0F1BEH9
• Locus tag: C1N69_RS02440
• Protein ID: WP_025759087.1
• Coordinates: 509713..509955 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1N69_RS02425	505808..506548	+	741	WP_155030610.1	KDGP aldolase family protein	-
C1N69_RS02430	506569..507705	+	1137	WP_155030611.1	lactonase family protein	-
C1N69_RS02435	507725..509635	+	1911	WP_155030612.1	BglG family transcription antiterminator	-
C1N69_RS02440	509713..509955	+	243	WP_025759087.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C1N69_RS02445	509945..510229	+	285	WP_025759089.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1N69_RS02450	510233..510697	-	465	WP_155030613.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
C1N69_RS02455	510839..512977	-	2139	WP_025759092.1	anaerobic ribonucleoside-triphosphate reductase	-
C1N69_RS02460	513354..514997	-	1644	WP_025759094.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10847.72 Da
• Isoelectric Point: 10.2746

>T100422 WP_025759089.1 NZ_CP027986:509945-510229 [Enterobacter sichuanensis]
MTYELVFDPRALKEWHKLGDTVKAQFKKKLAEVLAKPRNESARLHGLPDCYKIKLKASGYRLVYQVQDEVVKVVVIAIGK
REHSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T100422 NZ_CP027986:509945-510229 [Enterobacter sichuanensis]
ATGACTTATGAACTGGTATTTGACCCGAGGGCGTTAAAGGAGTGGCATAAGCTGGGTGATACGGTGAAAGCGCAGTTTAA
GAAAAAACTTGCCGAGGTGCTGGCGAAACCCAGGAACGAATCAGCTCGTTTACACGGCCTGCCTGATTGTTACAAAATAA
AACTTAAAGCGTCCGGCTATCGCCTGGTCTATCAGGTTCAGGACGAAGTAGTGAAAGTCGTTGTTATTGCCATTGGGAAA
AGAGAACATTCGGCCGTTTATCATGACGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8958.34 Da
• Isoelectric Point: 5.0651

>AT100422 WP_025759087.1 NZ_CP027986:509713-509955 [Enterobacter sichuanensis]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT100422 NZ_CP027986:509713-509955 [Enterobacter sichuanensis]
ATGGCCACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTACGCCGTGCTGGAAAAACTCAACATCACCCC
AACGGAAGCCGTGCGGTTACTGTTTCAATACGTTGCTGAAACGGGGCGCATGCCCGTGAAAACGGTCACGATTAGCGACA
GTGAAGATGCGCTGATACAGACGGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGCGTTAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0F1BEH9