Detailed information of TA system TA100421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 262908..263504
• Replicon: chromosome
• Accession: NZ_CP027986
• Organism: Enterobacter sichuanensis strain SGAir0282

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C1N69_RS01220
• Protein ID: WP_155030543.1
• Coordinates: 263202..263504 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A0F1AUP1
• Locus tag: C1N69_RS01215
• Protein ID: WP_013095028.1
• Coordinates: 262908..263195 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1N69_RS01210	261280..262911	+	1632	WP_025759001.1	Na/Pi cotransporter family protein	-
C1N69_RS01215	262908..263195	-	288	WP_013095028.1	putative addiction module antidote protein	Antitoxin
C1N69_RS01220	263202..263504	-	303	WP_155030543.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1N69_RS01225	263701..264573	+	873	WP_048957452.1	23S rRNA pseudouridine(2604) synthase RluF	-
C1N69_RS01230	264646..265590	-	945	WP_155030544.1	ketopantoate/pantoate/pantothenate transporter PanS	-
C1N69_RS01235	265684..267033	-	1350	WP_045286172.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11471.33 Da
• Isoelectric Point: 9.7662

>T100421 WP_155030543.1 NZ_CP027986:c263504-263202 [Enterobacter sichuanensis]
MKEIVQTESFRRWEQNLKDRRARTIIASRLFRLANGLAGDIKPVGEGICELRIHFGPGYRIYFKDQGNCIIVLLCGGEKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T100421 NZ_CP027986:c263504-263202 [Enterobacter sichuanensis]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGGCGAGCAAGGACTATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACATAAAACCCGTGGGGGAAGGTATATGCGAGCTGAGGATCC
ACTTTGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGGGAAAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10070.62 Da
• Isoelectric Point: 5.7807

>AT100421 WP_013095028.1 NZ_CP027986:c263195-262908 [Enterobacter sichuanensis]
MHKLTPYDPAIALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPAGD

Nucleotide

• Length: 288 bp

>AT100421 NZ_CP027986:c263195-262908 [Enterobacter sichuanensis]
ATGCATAAATTAACACCCTACGATCCAGCCATCGCGTTGGTGGATGATGAGGAAATAGCCGTGTTTATGGCTGATGCATT
AGAAACTGGTGACTCAGCGTATATTGCTAAAGCTTTGGGCGTCATCGCGCGAGCGAAAGGGATGTCGACCATTTCACAGC
AAACTGGCCTGTCACGAGAACAACTGTACCGATCATTTAGTGATAAGGGGAACCCAACGCTTAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTCACAATCAAACCTGCCGGGGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F1AUP1