Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 174602..175512 Replicon plasmid pCRKP18622-vir
Accession NZ_CP027867
Organism Klebsiella pneumoniae subsp. pneumoniae strain CRKP18622

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag C7K64_RS29450 Protein ID WP_004026354.1
Coordinates 175042..175512 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag C7K64_RS29445 Protein ID WP_004026357.1
Coordinates 174602..175045 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7K64_RS29415 (C7K64_30495) 169620..170141 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
C7K64_RS29420 (C7K64_30500) 170138..171091 + 954 Protein_181 fec operon regulator FecR -
C7K64_RS29425 (C7K64_30505) 171178..173304 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
C7K64_RS29430 (C7K64_30510) 173379..173498 + 120 Protein_183 Fe3+-citrate ABC transporter substrate-binding protein -
C7K64_RS29435 (C7K64_30515) 173679..173900 + 222 WP_004213643.1 DUF2188 domain-containing protein -
C7K64_RS29440 (C7K64_30520) 173998..174501 + 504 Protein_185 DUF4113 domain-containing protein -
C7K64_RS29445 (C7K64_30525) 174602..175045 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
C7K64_RS29450 (C7K64_30530) 175042..175512 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
C7K64_RS29455 (C7K64_30535) 175661..176358 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
C7K64_RS29460 (C7K64_30540) 177744..178394 - 651 WP_068893702.1 DUF1173 family protein -
C7K64_RS29465 178427..178693 - 267 WP_223175074.1 DUF1173 family protein -
C7K64_RS29470 (C7K64_30545) 178871..179365 + 495 WP_004212794.1 thermonuclease family protein -
C7K64_RS29475 (C7K64_30550) 179702..180100 + 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB 1..228553 228553
- flank IS/Tn - - 175855..176358 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T100418 WP_004026354.1 NZ_CP027867:175042-175512 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T100418 NZ_CP027867:175042-175512 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT100418 WP_004026357.1 NZ_CP027867:174602-175045 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT100418 NZ_CP027867:174602-175045 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References