Detailed information of TA system TA100416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 108750..109393
• Replicon: plasmid pCRKP18622-KPC
• Accession: NZ_CP027866
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain CRKP18622

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: V0UN72
• Locus tag: C7K64_RS28395
• Protein ID: WP_001034044.1
• Coordinates: 108977..109393 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B1P7N7
• Locus tag: C7K64_RS28390
• Protein ID: WP_001261286.1
• Coordinates: 108750..108980 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7K64_RS28350 (104056)	104056..104331	+	276	WP_000239529.1	hypothetical protein	-
C7K64_RS28355 (104523)	104523..104949	-	427	Protein_132	site-specific integrase	-
C7K64_RS28360 (105129)	105129..105719	+	591	Protein_133	colicin-B	-
C7K64_RS28370 (106488)	106488..106682	-	195	Protein_135	colicin M immunity protein	-
C7K64_RS28375 (106854)	106854..107663	-	810	WP_000016963.1	site-specific integrase	-
C7K64_RS28380 (107664)	107664..107969	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	-
C7K64_RS28385 (107971)	107971..108189	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
C7K64_RS28390 (108750)	108750..108980	+	231	WP_001261286.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
C7K64_RS28395 (108977)	108977..109393	+	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	Toxin
C7K64_RS28400 (109468)	109468..111033	+	1566	WP_001128474.1	AAA family ATPase	-
C7K64_RS28405 (111018)	111018..112040	+	1023	WP_000361402.1	helicase UvrD	-
C7K64_RS28410 (112294)	112294..112980	-	687	Protein_143	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B / blaKPC-2 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..134794	134794
	-	flank	IS/Tn	-	-	105735..106238	503
	-	flank	IS/Tn	-	-	112294..112641	347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.66 Da
• Isoelectric Point: 6.8536

>T100416 WP_001034044.1 NZ_CP027866:108977-109393 [Klebsiella pneumoniae subsp. pneumoniae]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T100416 NZ_CP027866:108977-109393 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8747.86 Da
• Isoelectric Point: 4.3775

>AT100416 WP_001261286.1 NZ_CP027866:108750-108980 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT100416 NZ_CP027866:108750-108980 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829CHW1

Antitoxin

• AlphaFold DB: A0A829CKZ6