Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 107664..108189 Replicon plasmid pCRKP18622-KPC
Accession NZ_CP027866
Organism Klebsiella pneumoniae subsp. pneumoniae strain CRKP18622

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C7K64_RS28380 Protein ID WP_001159871.1
Coordinates 107664..107969 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C7K64_RS28385 Protein ID WP_000813634.1
Coordinates 107971..108189 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7K64_RS28345 (103418) 103418..104062 + 645 WP_000633913.1 division plane positioning ATPase MipZ -
C7K64_RS28350 (104056) 104056..104331 + 276 WP_000239529.1 hypothetical protein -
C7K64_RS28355 (104523) 104523..104949 - 427 Protein_132 site-specific integrase -
C7K64_RS28360 (105129) 105129..105719 + 591 Protein_133 colicin-B -
C7K64_RS28370 (106488) 106488..106682 - 195 Protein_135 colicin M immunity protein -
C7K64_RS28375 (106854) 106854..107663 - 810 WP_000016963.1 site-specific integrase -
C7K64_RS28380 (107664) 107664..107969 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C7K64_RS28385 (107971) 107971..108189 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C7K64_RS28390 (108750) 108750..108980 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
C7K64_RS28395 (108977) 108977..109393 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
C7K64_RS28400 (109468) 109468..111033 + 1566 WP_001128474.1 AAA family ATPase -
C7K64_RS28405 (111018) 111018..112040 + 1023 WP_000361402.1 helicase UvrD -
C7K64_RS28410 (112294) 112294..112980 - 687 Protein_143 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B / blaKPC-2 / sitABCD iutA / iucD / iucC / iucB / iucA 1..134794 134794
- flank IS/Tn - - 105735..106238 503
- flank IS/Tn - - 112294..112641 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T100415 WP_001159871.1 NZ_CP027866:c107969-107664 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T100415 NZ_CP027866:c107969-107664 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT100415 WP_000813634.1 NZ_CP027866:c108189-107971 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT100415 NZ_CP027866:c108189-107971 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References