Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5389952..5390577 Replicon chromosome
Accession NZ_CP027863
Organism Klebsiella pneumoniae subsp. pneumoniae strain CRKP18622

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag C7K64_RS26020 Protein ID WP_012737091.1
Coordinates 5389952..5390335 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag C7K64_RS26025 Protein ID WP_004150355.1
Coordinates 5390335..5390577 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7K64_RS26005 (5387318) 5387318..5388220 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
C7K64_RS26010 (5388217) 5388217..5388852 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
C7K64_RS26015 (5388849) 5388849..5389778 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
C7K64_RS26020 (5389952) 5389952..5390335 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
C7K64_RS26025 (5390335) 5390335..5390577 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
C7K64_RS26030 (5390782) 5390782..5391699 + 918 WP_012737090.1 alpha/beta hydrolase -
C7K64_RS26035 (5391713) 5391713..5392654 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
C7K64_RS26040 (5392699) 5392699..5393136 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
C7K64_RS26045 (5393133) 5393133..5393993 - 861 WP_004146232.1 virulence factor BrkB family protein -
C7K64_RS26050 (5393987) 5393987..5394586 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T100405 WP_012737091.1 NZ_CP027863:c5390335-5389952 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T100405 NZ_CP027863:c5390335-5389952 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT100405 WP_004150355.1 NZ_CP027863:c5390577-5390335 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT100405 NZ_CP027863:c5390577-5390335 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References