Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4162960..4163579 Replicon chromosome
Accession NZ_CP027863
Organism Klebsiella pneumoniae subsp. pneumoniae strain CRKP18622

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C7K64_RS20205 Protein ID WP_002892050.1
Coordinates 4163361..4163579 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag C7K64_RS20200 Protein ID WP_002892066.1
Coordinates 4162960..4163334 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7K64_RS20190 (4158112) 4158112..4159305 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C7K64_RS20195 (4159328) 4159328..4162474 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
C7K64_RS20200 (4162960) 4162960..4163334 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
C7K64_RS20205 (4163361) 4163361..4163579 + 219 WP_002892050.1 HHA domain-containing protein Toxin
C7K64_RS20210 (4163738) 4163738..4164304 + 567 WP_002892030.1 maltose O-acetyltransferase -
C7K64_RS20215 (4164276) 4164276..4164416 - 141 WP_004147370.1 hypothetical protein -
C7K64_RS20220 (4164437) 4164437..4164907 + 471 WP_002892026.1 YlaC family protein -
C7K64_RS20225 (4164882) 4164882..4166333 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
C7K64_RS20230 (4166434) 4166434..4167132 + 699 WP_002892021.1 GNAT family protein -
C7K64_RS20235 (4167129) 4167129..4167269 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
C7K64_RS20240 (4167269) 4167269..4167532 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T100402 WP_002892050.1 NZ_CP027863:4163361-4163579 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T100402 NZ_CP027863:4163361-4163579 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT100402 WP_002892066.1 NZ_CP027863:4162960-4163334 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT100402 NZ_CP027863:4162960-4163334 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References