Detailed information of TA system TA100387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 2817510..2818065
• Replicon: chromosome
• Accession: NZ_CP027862
• Organism: Actinomyces sp. Z16

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C3B71_RS11795
• Protein ID: WP_108968268.1
• Coordinates: 2817510..2817833 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1M4RXV0
• Locus tag: C3B71_RS11800
• Protein ID: WP_073328490.1
• Coordinates: 2817820..2818065 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3B71_RS11775	2813675..2814199	-	525	WP_108968263.1	GNAT family N-acetyltransferase	-
C3B71_RS11780	2814246..2815064	-	819	WP_199912502.1	Fic family protein	-
C3B71_RS11785	2815123..2816190	-	1068	WP_199912503.1	low-specificity L-threonine aldolase	-
C3B71_RS11790	2816226..2817293	-	1068	WP_199912504.1	glutamine-hydrolyzing GMP synthase	-
C3B71_RS11795	2817510..2817833	-	324	WP_108968268.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C3B71_RS11800	2817820..2818065	-	246	WP_073328490.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C3B71_RS11805	2818163..2819719	-	1557	WP_108969881.1	glutamine-hydrolyzing GMP synthase	-
C3B71_RS11810	2819931..2820947	+	1017	WP_108968271.1	LacI family transcriptional regulator	-
C3B71_RS11815	2821047..2822663	-	1617	WP_108968273.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-58)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11778.62 Da
• Isoelectric Point: 5.1596

>T100387 WP_108968268.1 NZ_CP027862:c2817833-2817510 [Actinomyces sp. Z16]
MRRGEVWTLRDDAYASKARPVVVVQNDELTGFGSVVLCLFTSFDSRDIPTRVPIEPSEGNGLAKPSYVMTEKIVTVDKQL
LGKRVGILGKDDMAEVSRQLVVVLGLE

Nucleotide

• Length: 324 bp

>T100387 NZ_CP027862:c2817833-2817510 [Actinomyces sp. Z16]
ATGAGACGCGGTGAGGTTTGGACGCTGCGCGACGATGCCTACGCTAGCAAGGCGCGACCGGTCGTTGTCGTACAGAACGA
TGAGCTGACAGGCTTCGGATCGGTTGTCCTGTGCCTATTCACGAGCTTCGACTCGCGCGACATCCCCACGAGGGTCCCTA
TCGAGCCGAGTGAAGGCAATGGACTTGCGAAGCCGAGCTACGTGATGACCGAGAAGATCGTCACTGTGGACAAACAACTT
CTCGGAAAGCGAGTTGGCATCCTGGGCAAGGATGACATGGCCGAGGTCTCGCGGCAGTTGGTCGTCGTCTTGGGCCTGGA
ATAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9254.40 Da
• Isoelectric Point: 4.8096

>AT100387 WP_073328490.1 NZ_CP027862:c2818065-2817820 [Actinomyces sp. Z16]
MAMRQIATRVDAEQAELFKETTRKLGTTPADVLRMFVTAFNSHRGFPYDVRLSEDVEPFATEEDATRFATDLSLRAINET
R

Nucleotide

• Length: 246 bp

>AT100387 NZ_CP027862:c2818065-2817820 [Actinomyces sp. Z16]
ATGGCAATGAGACAGATCGCCACGAGGGTCGACGCGGAGCAGGCGGAGCTCTTCAAGGAGACCACGAGAAAGCTCGGCAC
TACCCCGGCGGACGTGCTGCGCATGTTCGTGACCGCCTTCAACAGTCACCGTGGCTTCCCCTACGACGTGCGCCTCTCGG
AGGACGTCGAGCCGTTCGCCACCGAGGAGGACGCCACCAGGTTTGCGACAGACCTCTCCCTGAGGGCGATCAATGAGACG
CGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1M4RXV0