Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4717758..4718366 Replicon chromosome
Accession NZ_CP027857
Organism Pseudomonas aeruginosa strain MPAO1

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag MPAO1_RS21800 Protein ID WP_003114156.1
Coordinates 4717758..4718105 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag MPAO1_RS21805 Protein ID WP_003114155.1
Coordinates 4718115..4718366 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPAO1_RS21770 4713117..4713389 + 273 WP_010895521.1 cysteine-rich CWC family protein -
MPAO1_RS21775 4713389..4714081 + 693 WP_003114159.1 16S rRNA pseudouridine(516) synthase -
MPAO1_RS21780 4714217..4715260 + 1044 WP_003098363.1 L,D-transpeptidase -
MPAO1_RS21785 4715340..4716077 + 738 WP_003114158.1 murein L,D-transpeptidase catalytic domain family protein -
MPAO1_RS21790 4716529..4717431 + 903 WP_003114157.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
MPAO1_RS21800 4717758..4718105 - 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MPAO1_RS21805 4718115..4718366 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MPAO1_RS21810 4718580..4719563 - 984 WP_003114154.1 tyrosine-type recombinase/integrase -
MPAO1_RS21815 4719563..4720855 - 1293 WP_003115206.1 hypothetical protein -
MPAO1_RS21820 4721085..4722359 - 1275 WP_010895520.1 zonular occludens toxin family protein -
MPAO1_RS21825 4722363..4722719 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4717758..4739942 22184


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T100381 WP_003114156.1 NZ_CP027857:c4718105-4717758 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T100381 NZ_CP027857:c4718105-4717758 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT100381 WP_003114155.1 NZ_CP027857:c4718366-4718115 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT100381 NZ_CP027857:c4718366-4718115 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References