Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/- |
| Location | 3380465..3382266 | Replicon | chromosome |
| Accession | CP000681 | ||
| Organism | Shewanella putrefaciens CN-32 | ||
Toxin (Protein)
| Gene name | hipA | Uniprot ID | - |
| Locus tag | Sputcn32_2916 | Protein ID | ABP76633.1 |
| Coordinates | 3380920..3382266 (-) | Length | 449 a.a. |
Antitoxin (Protein)
| Gene name | hipB | Uniprot ID | - |
| Locus tag | Sputcn32_2915 | Protein ID | ABP76632.1 |
| Coordinates | 3380465..3380923 (-) | Length | 153 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Sputcn32_2912 | 3375839..3378976 | + | 3138 | ABP76629.1 | type I site-specific deoxyribonuclease, HsdR family | - |
| Sputcn32_2913 | 3379039..3379461 | - | 423 | ABP76630.1 | transcriptional regulator, XRE family | - |
| Sputcn32_2914 | 3379625..3380281 | + | 657 | ABP76631.1 | conserved hypothetical protein | - |
| Sputcn32_2915 | 3380465..3380923 | - | 459 | ABP76632.1 | transcriptional regulator, XRE family | Antitoxin |
| Sputcn32_2916 | 3380920..3382266 | - | 1347 | ABP76633.1 | HipA domain protein | Toxin |
| Sputcn32_2917 | 3383263..3383625 | + | 363 | ABP76634.1 | conserved hypothetical protein | - |
| Sputcn32_2918 | 3383654..3384571 | - | 918 | ABP76635.1 | transposase, IS4 family | - |
| Sputcn32_2919 | 3384696..3385229 | - | 534 | ABP76636.1 | conserved hypothetical protein | - |
| Sputcn32_2920 | 3385240..3386826 | - | 1587 | ABP76637.1 | von Willebrand factor, type A | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | flank | IS/Tn | - | - | 3383654..3384571 | 917 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 449 a.a. Molecular weight: 50997.60 Da Isoelectric Point: 4.6127
>T10038 ABP76633.1 CP000681:c3382266-3380920 [Shewanella putrefaciens CN-32]
MEQLTIQAYINGQWSDIAQLSFPESEQGNYRLTSLDYEQTYALAELDKDDLHAVSLNHPVSLFFDDLGRPGWLRFLDDIM
PSGASRRYWLNYLDIADLTLDEQNFVLLKFGTIAPVGNLRVKESLPKRTAIADTLKFTVDDVRNRAADFLDYAQHQGAAA
GGATGAGGEAPKLLLRCDDEQNIWIDSYQDEPQNRDLHYLVKYPRGARLPIDCDILRAEYHYYHELAEMGFETIPTANMR
LEEGHSYPSLWLPRFDIYFNELGQQRYAMESVYSILGKGAGVTLDHEQTIRALIDKITHSHMVLEQGYQFDVRAFVIEWV
RRDLLNILFGNSDNHGRNTAFLRGDGWIRLAPIYDFAPMKADPEGIARTTKWEAPLEIGGEYDFQSIAEALADLVPAQEL
LAELKRTAQQCLGLEQRLIGRGVPQRIIDMPAIGFAYLEDKLTRWGLV
MEQLTIQAYINGQWSDIAQLSFPESEQGNYRLTSLDYEQTYALAELDKDDLHAVSLNHPVSLFFDDLGRPGWLRFLDDIM
PSGASRRYWLNYLDIADLTLDEQNFVLLKFGTIAPVGNLRVKESLPKRTAIADTLKFTVDDVRNRAADFLDYAQHQGAAA
GGATGAGGEAPKLLLRCDDEQNIWIDSYQDEPQNRDLHYLVKYPRGARLPIDCDILRAEYHYYHELAEMGFETIPTANMR
LEEGHSYPSLWLPRFDIYFNELGQQRYAMESVYSILGKGAGVTLDHEQTIRALIDKITHSHMVLEQGYQFDVRAFVIEWV
RRDLLNILFGNSDNHGRNTAFLRGDGWIRLAPIYDFAPMKADPEGIARTTKWEAPLEIGGEYDFQSIAEALADLVPAQEL
LAELKRTAQQCLGLEQRLIGRGVPQRIIDMPAIGFAYLEDKLTRWGLV
Download Length: 1347 bp
>T10038 CP000681:c3382266-3380920 [Shewanella putrefaciens CN-32]
ATGGAACAGTTGACCATTCAGGCGTACATTAATGGCCAATGGAGCGATATTGCACAGCTCTCATTCCCTGAGAGCGAACA
AGGTAATTATCGGCTTACCTCGTTAGATTACGAGCAAACATATGCATTAGCTGAGCTAGATAAAGACGACTTGCACGCCG
TCTCGCTAAACCATCCTGTTAGCCTTTTTTTCGACGATCTAGGTAGACCAGGATGGTTACGCTTTCTTGATGACATCATG
CCTAGCGGTGCAAGCCGGCGCTATTGGCTTAACTATTTAGATATCGCAGACCTGACCTTAGACGAGCAAAATTTTGTACT
TTTGAAGTTTGGGACCATCGCTCCTGTGGGGAATTTGAGGGTAAAGGAATCTTTGCCTAAGCGCACCGCTATCGCTGATA
CACTGAAATTCACCGTAGACGACGTTAGGAATCGAGCTGCTGACTTTTTGGATTATGCACAACATCAAGGCGCTGCCGCT
GGTGGCGCAACAGGGGCTGGCGGCGAAGCGCCCAAGCTTTTGTTACGTTGCGACGATGAACAAAATATCTGGATTGATAG
CTATCAAGACGAACCGCAAAACCGTGACCTTCATTATCTGGTGAAATATCCAAGAGGCGCTCGGTTGCCTATTGACTGCG
ATATATTACGCGCGGAATACCACTATTATCACGAATTGGCTGAAATGGGCTTTGAGACCATTCCTACGGCTAATATGCGG
CTTGAAGAAGGCCATTCCTACCCATCGCTATGGTTGCCGCGCTTTGATATTTACTTCAACGAACTAGGCCAGCAACGTTA
TGCGATGGAGTCGGTCTATTCGATTTTGGGTAAAGGCGCGGGCGTTACCTTAGATCATGAGCAAACCATAAGAGCGTTGA
TCGATAAAATCACTCATAGCCATATGGTCTTAGAGCAAGGTTATCAGTTTGATGTGCGTGCTTTTGTCATTGAGTGGGTG
CGGCGAGACTTACTCAATATCCTATTTGGCAATAGTGATAATCACGGGCGCAATACCGCGTTTCTTAGGGGCGATGGTTG
GATAAGGCTTGCGCCTATCTATGACTTTGCACCAATGAAAGCTGACCCTGAAGGCATAGCCAGAACCACAAAGTGGGAAG
CCCCGCTCGAAATAGGGGGGGAGTATGATTTCCAGAGCATTGCTGAGGCATTAGCTGATTTGGTGCCTGCTCAGGAGCTA
CTGGCAGAGTTAAAACGTACAGCACAGCAATGCCTTGGCTTAGAGCAACGCTTAATAGGGCGTGGTGTGCCACAGCGCAT
TATCGACATGCCAGCAATTGGCTTTGCTTACCTCGAAGACAAACTAACGCGTTGGGGATTGGTATGA
ATGGAACAGTTGACCATTCAGGCGTACATTAATGGCCAATGGAGCGATATTGCACAGCTCTCATTCCCTGAGAGCGAACA
AGGTAATTATCGGCTTACCTCGTTAGATTACGAGCAAACATATGCATTAGCTGAGCTAGATAAAGACGACTTGCACGCCG
TCTCGCTAAACCATCCTGTTAGCCTTTTTTTCGACGATCTAGGTAGACCAGGATGGTTACGCTTTCTTGATGACATCATG
CCTAGCGGTGCAAGCCGGCGCTATTGGCTTAACTATTTAGATATCGCAGACCTGACCTTAGACGAGCAAAATTTTGTACT
TTTGAAGTTTGGGACCATCGCTCCTGTGGGGAATTTGAGGGTAAAGGAATCTTTGCCTAAGCGCACCGCTATCGCTGATA
CACTGAAATTCACCGTAGACGACGTTAGGAATCGAGCTGCTGACTTTTTGGATTATGCACAACATCAAGGCGCTGCCGCT
GGTGGCGCAACAGGGGCTGGCGGCGAAGCGCCCAAGCTTTTGTTACGTTGCGACGATGAACAAAATATCTGGATTGATAG
CTATCAAGACGAACCGCAAAACCGTGACCTTCATTATCTGGTGAAATATCCAAGAGGCGCTCGGTTGCCTATTGACTGCG
ATATATTACGCGCGGAATACCACTATTATCACGAATTGGCTGAAATGGGCTTTGAGACCATTCCTACGGCTAATATGCGG
CTTGAAGAAGGCCATTCCTACCCATCGCTATGGTTGCCGCGCTTTGATATTTACTTCAACGAACTAGGCCAGCAACGTTA
TGCGATGGAGTCGGTCTATTCGATTTTGGGTAAAGGCGCGGGCGTTACCTTAGATCATGAGCAAACCATAAGAGCGTTGA
TCGATAAAATCACTCATAGCCATATGGTCTTAGAGCAAGGTTATCAGTTTGATGTGCGTGCTTTTGTCATTGAGTGGGTG
CGGCGAGACTTACTCAATATCCTATTTGGCAATAGTGATAATCACGGGCGCAATACCGCGTTTCTTAGGGGCGATGGTTG
GATAAGGCTTGCGCCTATCTATGACTTTGCACCAATGAAAGCTGACCCTGAAGGCATAGCCAGAACCACAAAGTGGGAAG
CCCCGCTCGAAATAGGGGGGGAGTATGATTTCCAGAGCATTGCTGAGGCATTAGCTGATTTGGTGCCTGCTCAGGAGCTA
CTGGCAGAGTTAAAACGTACAGCACAGCAATGCCTTGGCTTAGAGCAACGCTTAATAGGGCGTGGTGTGCCACAGCGCAT
TATCGACATGCCAGCAATTGGCTTTGCTTACCTCGAAGACAAACTAACGCGTTGGGGATTGGTATGA
Antitoxin
Download Length: 153 a.a. Molecular weight: 16944.30 Da Isoelectric Point: 10.6618
>AT10038 ABP76632.1 CP000681:c3380923-3380465 [Shewanella putrefaciens CN-32]
MSDKQTTTETAKDTIARLRANRQQQSDIAIEASQHRQPVNIGEAQRKQDAKRASKQARGVSTVERKNVIYSTAQKLLHGE
LTQGQALKALRVEVLGLTQDEYANLVKVSRKTLSDVENDRGSYKTDILNRLFKPFGLKVGIVPISTGVTWLL
MSDKQTTTETAKDTIARLRANRQQQSDIAIEASQHRQPVNIGEAQRKQDAKRASKQARGVSTVERKNVIYSTAQKLLHGE
LTQGQALKALRVEVLGLTQDEYANLVKVSRKTLSDVENDRGSYKTDILNRLFKPFGLKVGIVPISTGVTWLL
Download Length: 459 bp
>AT10038 CP000681:c3380923-3380465 [Shewanella putrefaciens CN-32]
ATGAGTGATAAACAAACGACTACTGAAACGGCTAAAGATACCATCGCCCGTCTTAGAGCCAATCGCCAGCAACAGAGTGA
TATTGCGATAGAGGCGTCACAACATCGGCAACCCGTAAATATTGGAGAGGCTCAAAGAAAGCAAGATGCTAAACGAGCAT
CAAAGCAAGCGAGGGGCGTTAGCACGGTCGAGCGCAAAAATGTGATTTATTCAACCGCACAAAAGCTGCTACATGGTGAG
TTAACCCAAGGACAGGCACTTAAAGCCCTGAGAGTTGAGGTGTTAGGACTCACACAAGATGAGTATGCAAACTTGGTTAA
AGTATCGCGCAAGACCCTCTCGGACGTTGAGAATGACCGTGGAAGCTATAAGACAGACATTCTGAATCGATTATTTAAGC
CCTTTGGTCTTAAGGTCGGGATTGTGCCGATCTCTACAGGTGTCACATGGCTTTTATGA
ATGAGTGATAAACAAACGACTACTGAAACGGCTAAAGATACCATCGCCCGTCTTAGAGCCAATCGCCAGCAACAGAGTGA
TATTGCGATAGAGGCGTCACAACATCGGCAACCCGTAAATATTGGAGAGGCTCAAAGAAAGCAAGATGCTAAACGAGCAT
CAAAGCAAGCGAGGGGCGTTAGCACGGTCGAGCGCAAAAATGTGATTTATTCAACCGCACAAAAGCTGCTACATGGTGAG
TTAACCCAAGGACAGGCACTTAAAGCCCTGAGAGTTGAGGTGTTAGGACTCACACAAGATGAGTATGCAAACTTGGTTAA
AGTATCGCGCAAGACCCTCTCGGACGTTGAGAATGACCGTGGAAGCTATAAGACAGACATTCTGAATCGATTATTTAAGC
CCTTTGGTCTTAAGGTCGGGATTGTGCCGATCTCTACAGGTGTCACATGGCTTTTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Yi Zhao et al. (2022) The HipAB Toxin-Antitoxin System Stabilizes a Composite Genomic Island in Shewanella putrefaciens CN-32. Frontiers in Microbiology 13:858857. [PubMed:35387082]