Detailed information of TA system TA100372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 939506..940030
• Replicon: chromosome
• Accession: NZ_CP027848
• Organism: Staphylococcus muscae strain ATCC 49910

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C7J88_RS04650
• Protein ID: WP_095117482.1
• Coordinates: 939506..939859 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C7J88_RS04655
• Protein ID: WP_095117483.1
• Coordinates: 939860..940030 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7J88_RS04630	936575..937348	-	774	WP_095117478.1	RNA polymerase sigma factor SigB	-
C7J88_RS04635	937320..937802	-	483	WP_095117479.1	anti-sigma B factor RsbW	-
C7J88_RS04640	937804..938130	-	327	WP_095117480.1	anti-sigma factor antagonist	-
C7J88_RS04645	938206..939234	-	1029	WP_095117481.1	PP2C family protein-serine/threonine phosphatase	-
C7J88_RS04650	939506..939859	-	354	WP_095117482.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C7J88_RS04655	939860..940030	-	171	WP_095117483.1	antitoxin MazE	Antitoxin
C7J88_RS04660	940119..941267	-	1149	WP_095117484.1	alanine racemase	-
C7J88_RS04665	941295..941654	-	360	WP_095117485.1	holo-ACP synthase	-
C7J88_RS04670	941651..942226	-	576	WP_095117486.1	PH domain-containing protein	-
C7J88_RS04675	942216..943721	-	1506	WP_095117487.1	PH domain-containing protein	-
C7J88_RS04680	943714..944187	-	474	WP_095117488.1	PH domain-containing protein	-
C7J88_RS04685	944322..945023	-	702	WP_095117489.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13030.17 Da
• Isoelectric Point: 10.2682

>T100372 WP_095117482.1 NZ_CP027848:c939859-939506 [Staphylococcus muscae]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRT
VDKNRLKEKLTFLSDEKMKEVNNALGISLGLQVVQSR

Nucleotide

• Length: 354 bp

>T100372 NZ_CP027848:c939859-939506 [Staphylococcus muscae]
ATGAAGCGCGGTGATGTATACTTAGCTGATTTATCACCAGTACAGGGTTCTGAACAAGGGGGAGTTAGACCCGTCGTAAT
CATACAAAATGATACTGGTAACAAATATAGTCCGACTGTGATTGTAGCGGCAATTACTGGACGTATTAATAAAGCGAAGA
TTCCAACACATGTAGAAATTGAAAAAAAGAAATACAAACTGGACAAAGATTCTGTTATTTTGTTAGAACAGATTCGGACA
GTTGATAAAAATCGATTGAAAGAAAAACTGACTTTTTTATCGGACGAAAAGATGAAAGAAGTAAACAATGCACTCGGTAT
CAGTCTAGGGTTACAAGTGGTTCAATCACGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6327.02 Da
• Isoelectric Point: 4.2313

>AT100372 WP_095117483.1 NZ_CP027848:c940030-939860 [Staphylococcus muscae]
MSVFNQVKFKNLEQSLKEGYAQMADLNLSLATEAYSVECEACDCNESHLLSKHQDD

Nucleotide

• Length: 171 bp

>AT100372 NZ_CP027848:c940030-939860 [Staphylococcus muscae]
ATGTCAGTATTCAATCAAGTAAAATTTAAAAATTTAGAGCAGTCATTAAAAGAAGGTTATGCACAAATGGCTGATCTCAA
TCTCTCCCTAGCGACAGAAGCATATTCAGTAGAGTGTGAGGCGTGCGATTGTAATGAATCTCACTTACTATCAAAGCATC
AGGATGATTAA