Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 189045..189955 Replicon plasmid pVH1-2-VIR
Accession NZ_CP027803
Organism Klebsiella pneumoniae subsp. pneumoniae strain VH1-2

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag C7K65_RS28975 Protein ID WP_004026354.1
Coordinates 189485..189955 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag C7K65_RS28970 Protein ID WP_004026357.1
Coordinates 189045..189488 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7K65_RS28940 (C7K65_29995) 184063..184584 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
C7K65_RS28945 (C7K65_30000) 184581..185534 + 954 Protein_225 fec operon regulator FecR -
C7K65_RS28950 (C7K65_30005) 185621..187747 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
C7K65_RS28955 (C7K65_30010) 187822..187941 + 120 Protein_227 Fe3+-citrate ABC transporter substrate-binding protein -
C7K65_RS28960 (C7K65_30015) 188122..188343 + 222 WP_004213643.1 DUF2188 domain-containing protein -
C7K65_RS28965 (C7K65_30020) 188441..188944 + 504 Protein_229 DUF4113 domain-containing protein -
C7K65_RS28970 (C7K65_30025) 189045..189488 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
C7K65_RS28975 (C7K65_30030) 189485..189955 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
C7K65_RS28980 (C7K65_30035) 190104..190801 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
C7K65_RS28985 (C7K65_30040) 192187..192837 - 651 WP_068893702.1 DUF1173 family protein -
C7K65_RS28990 192870..193136 - 267 WP_223175074.1 DUF1173 family protein -
C7K65_RS28995 (C7K65_30045) 193314..193808 + 495 WP_004212794.1 thermonuclease family protein -
C7K65_RS29000 (C7K65_30050) 194145..194543 + 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / qnrB6 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1C rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB 1..346781 346781


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T100359 WP_004026354.1 NZ_CP027803:189485-189955 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T100359 NZ_CP027803:189485-189955 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT100359 WP_004026357.1 NZ_CP027803:189045-189488 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT100359 NZ_CP027803:189045-189488 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References