Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 133219..133744 | Replicon | plasmid pVH1-2-VIR |
| Accession | NZ_CP027803 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain VH1-2 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | D4HQE7 |
| Locus tag | C7K65_RS28640 | Protein ID | WP_013023785.1 |
| Coordinates | 133219..133524 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | W8V2V6 |
| Locus tag | C7K65_RS28645 | Protein ID | WP_001568025.1 |
| Coordinates | 133526..133744 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C7K65_RS28610 (C7K65_29640) | 128938..129564 | + | 627 | WP_004197649.1 | ParA family plasmid-partitioning AAA ATPase | - |
| C7K65_RS28615 | 129561..129863 | + | 303 | WP_004197636.1 | hypothetical protein | - |
| C7K65_RS28620 (C7K65_29650) | 130295..131089 | - | 795 | WP_004197635.1 | site-specific integrase | - |
| C7K65_RS28625 (C7K65_29655) | 131287..132303 | - | 1017 | WP_017899884.1 | hypothetical protein | - |
| C7K65_RS28630 (C7K65_29660) | 132314..132628 | - | 315 | WP_053389906.1 | hypothetical protein | - |
| C7K65_RS28635 (C7K65_29665) | 132655..133050 | - | 396 | WP_017899885.1 | hypothetical protein | - |
| C7K65_RS28640 (C7K65_29670) | 133219..133524 | - | 306 | WP_013023785.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| C7K65_RS28645 (C7K65_29675) | 133526..133744 | - | 219 | WP_001568025.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| C7K65_RS28650 (C7K65_29685) | 133914..134177 | - | 264 | Protein_166 | hypothetical protein | - |
| C7K65_RS28655 (C7K65_29690) | 134386..134592 | + | 207 | WP_004213077.1 | hypothetical protein | - |
| C7K65_RS28660 (C7K65_29695) | 134582..134875 | - | 294 | WP_004213076.1 | hypothetical protein | - |
| C7K65_RS28665 (C7K65_29700) | 134891..136024 | - | 1134 | WP_004213075.1 | DUF3800 domain-containing protein | - |
| C7K65_RS28670 (C7K65_29705) | 136632..136862 | + | 231 | WP_004213073.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| C7K65_RS28675 (C7K65_29710) | 136859..137302 | + | 444 | WP_004213072.1 | type II toxin-antitoxin system VapC family toxin | - |
| C7K65_RS28680 | 137451..137702 | + | 252 | WP_186987481.1 | hypothetical protein | - |
| C7K65_RS28685 (C7K65_29720) | 137725..138029 | - | 305 | Protein_173 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | mph(A) / sul1 / qacE / qnrB6 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1C | rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB | 1..346781 | 346781 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11615.30 Da Isoelectric Point: 6.4672
>T100357 WP_013023785.1 NZ_CP027803:c133524-133219 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T100357 NZ_CP027803:c133524-133219 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8392.34 Da Isoelectric Point: 4.7405
>AT100357 WP_001568025.1 NZ_CP027803:c133744-133526 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT100357 NZ_CP027803:c133744-133526 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZP8 |