Detailed information of TA system TA100356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 108926..109569
• Replicon: plasmid pVH1-2-KPC
• Accession: NZ_CP027802
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain VH1-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: V0UN72
• Locus tag: C7K65_RS27720
• Protein ID: WP_001034044.1
• Coordinates: 109153..109569 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B1P7N7
• Locus tag: C7K65_RS27715
• Protein ID: WP_001261286.1
• Coordinates: 108926..109156 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7K65_RS27675 (104232)	104232..104507	+	276	WP_000239529.1	hypothetical protein	-
C7K65_RS27680 (104699)	104699..105125	-	427	Protein_130	site-specific integrase	-
C7K65_RS27685 (105305)	105305..105895	+	591	Protein_131	colicin-B	-
C7K65_RS27695 (106664)	106664..106858	-	195	Protein_133	colicin M immunity protein	-
C7K65_RS27700 (107030)	107030..107839	-	810	WP_000016963.1	site-specific integrase	-
C7K65_RS27705 (107840)	107840..108145	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	-
C7K65_RS27710 (108147)	108147..108365	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
C7K65_RS27715 (108926)	108926..109156	+	231	WP_001261286.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
C7K65_RS27720 (109153)	109153..109569	+	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	Toxin
C7K65_RS27725 (109644)	109644..111209	+	1566	WP_001128474.1	AAA family ATPase	-
C7K65_RS27730 (111194)	111194..112216	+	1023	WP_000361402.1	helicase UvrD	-
C7K65_RS27735 (112470)	112470..113156	-	687	Protein_141	IS1 family transposase	-
C7K65_RS27740 (113211)	113211..114371	-	1161	Protein_142	ferric aerobactin receptor IutA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	cmlA1 / blaTEM-1B / blaKPC-2 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..128932	128932
	-	flank	IS/Tn	-	-	105911..106414	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.66 Da
• Isoelectric Point: 6.8536

>T100356 WP_001034044.1 NZ_CP027802:109153-109569 [Klebsiella pneumoniae subsp. pneumoniae]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T100356 NZ_CP027802:109153-109569 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8747.86 Da
• Isoelectric Point: 4.3775

>AT100356 WP_001261286.1 NZ_CP027802:108926-109156 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT100356 NZ_CP027802:108926-109156 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829CHW1

Antitoxin

• AlphaFold DB: A0A829CKZ6