Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 107840..108365 Replicon plasmid pVH1-2-KPC
Accession NZ_CP027802
Organism Klebsiella pneumoniae subsp. pneumoniae strain VH1-2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C7K65_RS27705 Protein ID WP_001159871.1
Coordinates 107840..108145 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C7K65_RS27710 Protein ID WP_000813634.1
Coordinates 108147..108365 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7K65_RS27670 (103594) 103594..104238 + 645 WP_000633913.1 division plane positioning ATPase MipZ -
C7K65_RS27675 (104232) 104232..104507 + 276 WP_000239529.1 hypothetical protein -
C7K65_RS27680 (104699) 104699..105125 - 427 Protein_130 site-specific integrase -
C7K65_RS27685 (105305) 105305..105895 + 591 Protein_131 colicin-B -
C7K65_RS27695 (106664) 106664..106858 - 195 Protein_133 colicin M immunity protein -
C7K65_RS27700 (107030) 107030..107839 - 810 WP_000016963.1 site-specific integrase -
C7K65_RS27705 (107840) 107840..108145 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C7K65_RS27710 (108147) 108147..108365 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C7K65_RS27715 (108926) 108926..109156 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
C7K65_RS27720 (109153) 109153..109569 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
C7K65_RS27725 (109644) 109644..111209 + 1566 WP_001128474.1 AAA family ATPase -
C7K65_RS27730 (111194) 111194..112216 + 1023 WP_000361402.1 helicase UvrD -
C7K65_RS27735 (112470) 112470..113156 - 687 Protein_141 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cmlA1 / blaTEM-1B / blaKPC-2 / sitABCD iutA / iucD / iucC / iucB / iucA 1..128932 128932
- flank IS/Tn - - 105911..106414 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T100355 WP_001159871.1 NZ_CP027802:c108145-107840 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T100355 NZ_CP027802:c108145-107840 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT100355 WP_000813634.1 NZ_CP027802:c108365-108147 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT100355 NZ_CP027802:c108365-108147 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References