Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 26020..26579 | Replicon | plasmid pVH1-2-KPC |
| Accession | NZ_CP027802 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain VH1-2 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | S1PMX5 |
| Locus tag | C7K65_RS27180 | Protein ID | WP_000421257.1 |
| Coordinates | 26020..26295 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | C7K65_RS27185 | Protein ID | WP_001178089.1 |
| Coordinates | 26295..26579 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C7K65_RS27140 (21193) | 21193..21382 | - | 190 | Protein_22 | Tn3 family transposase | - |
| C7K65_RS27145 (21712) | 21712..22572 | - | 861 | WP_000027057.1 | broad-spectrum class A beta-lactamase TEM-1 | - |
| C7K65_RS27150 (22755) | 22755..23153 | - | 399 | Protein_24 | recombinase family protein | - |
| C7K65_RS27155 (23216) | 23216..23920 | + | 705 | WP_001067845.1 | IS6-like element IS26 family transposase | - |
| C7K65_RS27160 (24007) | 24007..24354 | - | 348 | WP_000142451.1 | DUF6404 family protein | - |
| C7K65_RS27165 (24483) | 24483..24818 | + | 336 | WP_001080730.1 | colicin 1A immunity protein | - |
| C7K65_RS27170 (24840) | 24840..25208 | - | 369 | Protein_28 | colicin-like pore-forming protein | - |
| C7K65_RS27175 (25273) | 25273..25970 | + | 698 | WP_095033700.1 | IS1-like element IS1B family transposase | - |
| C7K65_RS27180 (26020) | 26020..26295 | - | 276 | WP_000421257.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| C7K65_RS27185 (26295) | 26295..26579 | - | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| C7K65_RS27190 (27090) | 27090..27794 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| C7K65_RS27195 (28200) | 28200..28817 | + | 618 | Protein_33 | replication protein | - |
| C7K65_RS27200 (29351) | 29351..29629 | + | 279 | WP_013213990.1 | hypothetical protein | - |
| C7K65_RS27205 (29740) | 29740..30165 | + | 426 | WP_013213989.1 | antirestriction protein | - |
| C7K65_RS27210 (30293) | 30293..30448 | + | 156 | WP_013213988.1 | hypothetical protein | - |
| C7K65_RS27215 (30494) | 30494..30790 | + | 297 | WP_013213987.1 | transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | cmlA1 / blaTEM-1B / blaKPC-2 / sitABCD | iutA / iucD / iucC / iucB / iucA | 1..128932 | 128932 | |
| - | inside | IScluster/Tn | cmlA1 / blaTEM-1B / blaKPC-2 | - | 97..38160 | 38063 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10749.45 Da Isoelectric Point: 9.9324
>T100347 WP_000421257.1 NZ_CP027802:c26295-26020 [Klebsiella pneumoniae subsp. pneumoniae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T100347 NZ_CP027802:c26295-26020 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT100347 WP_001178089.1 NZ_CP027802:c26579-26295 [Klebsiella pneumoniae subsp. pneumoniae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT100347 NZ_CP027802:c26579-26295 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1PMX5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |