Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4920361..4920877 Replicon chromosome
Accession NZ_CP027800
Organism Klebsiella pneumoniae subsp. pneumoniae strain VH1-2

Toxin (Protein)


Gene name relE Uniprot ID R4YAY3
Locus tag C7K65_RS23800 Protein ID WP_004178374.1
Coordinates 4920361..4920645 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag C7K65_RS23805 Protein ID WP_002886901.1
Coordinates 4920635..4920877 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7K65_RS23775 (4915757) 4915757..4916020 - 264 WP_014908078.1 PTS sugar transporter subunit IIB -
C7K65_RS23780 (4916150) 4916150..4916323 + 174 WP_041169004.1 hypothetical protein -
C7K65_RS23785 (4916326) 4916326..4917069 + 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
C7K65_RS23790 (4917426) 4917426..4919564 + 2139 WP_004186701.1 anaerobic ribonucleoside-triphosphate reductase -
C7K65_RS23795 (4919893) 4919893..4920357 + 465 WP_004222151.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
C7K65_RS23800 (4920361) 4920361..4920645 - 285 WP_004178374.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C7K65_RS23805 (4920635) 4920635..4920877 - 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
C7K65_RS23810 (4920955) 4920955..4922865 - 1911 WP_012737231.1 PRD domain-containing protein -
C7K65_RS23815 (4922888) 4922888..4924042 - 1155 WP_012737230.1 lactonase family protein -
C7K65_RS23820 (4924109) 4924109..4924849 - 741 WP_002886899.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11155.97 Da        Isoelectric Point: 10.3787

>T100345 WP_004178374.1 NZ_CP027800:c4920645-4920361 [Klebsiella pneumoniae subsp. pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T100345 NZ_CP027800:c4920645-4920361 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT100345 WP_002886901.1 NZ_CP027800:c4920877-4920635 [Klebsiella pneumoniae subsp. pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT100345 NZ_CP027800:c4920877-4920635 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6THG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References