Detailed information of TA system TA100328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 20674..21266
• Replicon: plasmid pTAB7A
• Accession: NZ_CP027790
• Organism: Bacillus licheniformis strain TAB7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C7M53_RS22990
• Protein ID: WP_119953980.1
• Coordinates: 20943..21266 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C7M53_RS22985
• Protein ID: WP_048406251.1
• Coordinates: 20674..20943 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M53_RS22955	15804..16112	+	309	WP_017474131.1	hypothetical protein	-
C7M53_RS22960	16109..16363	+	255	WP_017474130.1	hypothetical protein	-
C7M53_RS22965	16793..17122	-	330	WP_119953974.1	YolD-like family protein	-
C7M53_RS22970	17650..17856	+	207	WP_048406504.1	hypothetical protein	-
C7M53_RS23655	18230..18400	+	171	WP_164504587.1	hypothetical protein	-
C7M53_RS22975	19027..19959	+	933	WP_119953976.1	tyrosine-type recombinase/integrase	-
C7M53_RS22980	20170..20355	+	186	WP_119953978.1	hypothetical protein	-
C7M53_RS22985	20674..20943	+	270	WP_048406251.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C7M53_RS22990	20943..21266	+	324	WP_119953980.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C7M53_RS22995	21705..22805	+	1101	WP_119953982.1	tetratricopeptide repeat protein	-
C7M53_RS23000	23003..23185	-	183	WP_119953984.1	hypothetical protein	-
C7M53_RS23010	23856..24881	-	1026	WP_017474177.1	DUF2325 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42138	42138

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-96)

Antitoxin

• SpoVT-AbrB domain (17-61)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12002.70 Da
• Isoelectric Point: 5.6376

>T100328 WP_119953980.1 NZ_CP027790:20943-21266 [Bacillus licheniformis]
MSVERGDLVYLNFNPQAGHEQAGRRPAIVLSPKNFNQTTGFAVVCPITNQQKSYPFEVALPDGLAIQGVILTDQVKSLDW
RARRFQVVDQAPEIIVSECLDYIHTFL

Nucleotide

• Length: 324 bp

>T100328 NZ_CP027790:20943-21266 [Bacillus licheniformis]
ATGTCAGTTGAACGTGGTGATCTTGTTTATTTAAACTTTAATCCACAGGCAGGACATGAACAAGCTGGAAGAAGGCCAGC
TATTGTCTTATCTCCGAAAAATTTTAATCAAACCACTGGGTTTGCAGTGGTCTGTCCGATCACTAACCAACAGAAAAGTT
ATCCCTTTGAGGTAGCTTTGCCTGATGGCTTGGCTATCCAAGGGGTTATATTAACAGATCAGGTGAAAAGTTTGGATTGG
AGAGCCCGCCGGTTCCAAGTTGTAGATCAAGCTCCCGAAATAATTGTTAGTGAATGCTTGGACTATATACATACTTTTCT
ATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9939.27 Da
• Isoelectric Point: 5.1667

>AT100328 WP_048406251.1 NZ_CP027790:20674-20943 [Bacillus licheniformis]
MEENKREGVVSVTTTVQKWGNSLAVRIPTRIAESISMEQGTEIELTVGKTGIMLKPKNNKPTLDDLLARVTPENRHSEVD
FGREGNELF

Nucleotide

• Length: 270 bp

>AT100328 NZ_CP027790:20674-20943 [Bacillus licheniformis]
ATGGAGGAAAATAAACGAGAAGGAGTGGTGAGTGTGACAACAACGGTTCAAAAATGGGGGAACAGCCTTGCCGTTCGCAT
CCCTACTCGTATTGCTGAATCTATCTCCATGGAACAAGGCACAGAGATCGAATTAACTGTCGGAAAGACTGGGATTATGT
TAAAACCTAAAAATAATAAACCAACATTAGATGACCTTTTAGCACGCGTAACACCCGAAAATCGGCATTCTGAAGTGGAT
TTTGGCCGTGAAGGGAATGAATTATTTTAA