Detailed information of TA system
Overview
TA module
| Type | VII | Classification (family/domain) | HepT-MntA/- |
| Location | 3409..4316 | Replicon | chromosome |
| Accession | NZ_JSDP01000312 | ||
| Organism | Aphanizomenon flos-aquae 2012/KM1/D3 spades_34_len_T:34268 | ||
Toxin (Protein)
| Gene name | hepT | Uniprot ID | - |
| Locus tag | OA07_RS26470 | Protein ID | WP_052150109.1 |
| Coordinates | 3873..4316 (+) | Length | 148 a.a. |
Antitoxin (Protein)
| Gene name | mntA | Uniprot ID | - |
| Locus tag | OA07_RS26465 | Protein ID | WP_235335441.1 |
| Coordinates | 3409..3876 (+) | Length | 156 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OA07_RS26430 (OA07_26415) | 1..294 | + | 294 | Protein_0 | potassium-transporting ATPase subunit B | - |
| OA07_RS26435 (OA07_26420) | 458..652 | + | 195 | WP_039205087.1 | potassium-transporting ATPase subunit F | - |
| OA07_RS26440 (OA07_26425) | 652..1266 | + | 615 | WP_039205089.1 | K(+)-transporting ATPase subunit C | - |
| OA07_RS26445 (OA07_26430) | 1249..1575 | - | 327 | WP_052150108.1 | hypothetical protein | - |
| OA07_RS26450 (OA07_26435) | 1805..2671 | - | 867 | WP_039205091.1 | WYL domain-containing protein | - |
| OA07_RS26455 (OA07_26440) | 2782..3009 | + | 228 | WP_039205093.1 | hypothetical protein | - |
| OA07_RS26460 (OA07_26445) | 3157..3393 | + | 237 | WP_039205095.1 | DUF433 domain-containing protein | - |
| OA07_RS26465 (OA07_26450) | 3409..3876 | + | 468 | WP_235335441.1 | type VII toxin-antitoxin system antitoxin protein adenylyltransferase MntA | Antitoxin |
| OA07_RS26470 (OA07_26455) | 3873..4316 | + | 444 | WP_052150109.1 | type VII toxin-antitoxin system toxin tRNA nuclease HepT | Toxin |
| OA07_RS26475 (OA07_26460) | 4309..7005 | + | 2697 | WP_039205098.1 | CRISPR-associated protein Csc3 | - |
| OA07_RS26480 (OA07_26465) | 7034..8059 | + | 1026 | WP_039205100.1 | type I-D CRISPR-associated protein Cas7/Csc2 | - |
| OA07_RS26485 (OA07_26470) | 8063..8821 | + | 759 | WP_039205101.1 | type I-D CRISPR-associated protein Cas5/Csc1 | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 148 a.a. Molecular weight: 17109.52 Da Isoelectric Point: 4.4587
>T10031 WP_052150109.1 NZ_JSDP01000312:3873-4316 [Aphanizomenon flos-aquae 2012/KM1/D3]
MTNIEPVIIETRLELIGRYLDHLKKFENISLDDYLSSFEQQLITERLLQLITQAAIDINDHILSKLKSGKSYTNFEAFIE
LGKYQILTPELAKQIAPSSGLRNRLVHEYDDIDPNQVFMAISFALQQYPLYVRQINSYLITLEEEND
MTNIEPVIIETRLELIGRYLDHLKKFENISLDDYLSSFEQQLITERLLQLITQAAIDINDHILSKLKSGKSYTNFEAFIE
LGKYQILTPELAKQIAPSSGLRNRLVHEYDDIDPNQVFMAISFALQQYPLYVRQINSYLITLEEEND
Download Length: 444 bp
>T10031 NZ_JSDP01000312:3873-4316 [Aphanizomenon flos-aquae 2012/KM1/D3]
ATGACAAATATAGAACCTGTGATTATTGAAACTAGACTGGAGTTGATAGGTCGTTATCTTGATCACCTCAAAAAATTTGA
AAATATTAGTTTAGATGATTATTTGAGTAGTTTTGAACAGCAATTAATTACAGAAAGACTATTGCAATTAATAACTCAAG
CTGCAATAGACATCAATGATCATATATTGTCAAAACTCAAATCTGGAAAGTCTTACACTAATTTTGAGGCTTTCATTGAA
TTGGGTAAATATCAAATTCTTACTCCCGAACTAGCAAAACAGATTGCACCATCATCAGGTTTAAGAAATCGTTTGGTTCA
TGAATATGATGATATAGATCCGAATCAGGTTTTCATGGCAATTAGTTTTGCGTTACAGCAATATCCGCTTTATGTTAGAC
AAATCAACTCTTATTTAATTACATTGGAAGAAGAAAATGATTAA
ATGACAAATATAGAACCTGTGATTATTGAAACTAGACTGGAGTTGATAGGTCGTTATCTTGATCACCTCAAAAAATTTGA
AAATATTAGTTTAGATGATTATTTGAGTAGTTTTGAACAGCAATTAATTACAGAAAGACTATTGCAATTAATAACTCAAG
CTGCAATAGACATCAATGATCATATATTGTCAAAACTCAAATCTGGAAAGTCTTACACTAATTTTGAGGCTTTCATTGAA
TTGGGTAAATATCAAATTCTTACTCCCGAACTAGCAAAACAGATTGCACCATCATCAGGTTTAAGAAATCGTTTGGTTCA
TGAATATGATGATATAGATCCGAATCAGGTTTTCATGGCAATTAGTTTTGCGTTACAGCAATATCCGCTTTATGTTAGAC
AAATCAACTCTTATTTAATTACATTGGAAGAAGAAAATGATTAA
Antitoxin
Download Length: 156 a.a. Molecular weight: 17930.66 Da Isoelectric Point: 5.0501
>AT10031 WP_235335441.1 NZ_JSDP01000312:3409-3876 [Aphanizomenon flos-aquae 2012/KM1/D3]
VDNDKMQDKIPTIAELRELSLRLLTKIPYLKMLVLFGSRATGNINANSDWDFAVLYDEEKYNLYIQNNPLAAFVIPGILG
EIFKINSDKIDIVELNHCSKLIAHFVARDGKVLYEEPGDEFDKFQQRVLLSNTEIKKIEKTKLENIENFLQRWGV
VDNDKMQDKIPTIAELRELSLRLLTKIPYLKMLVLFGSRATGNINANSDWDFAVLYDEEKYNLYIQNNPLAAFVIPGILG
EIFKINSDKIDIVELNHCSKLIAHFVARDGKVLYEEPGDEFDKFQQRVLLSNTEIKKIEKTKLENIENFLQRWGV
Download Length: 468 bp
>AT10031 NZ_JSDP01000312:3409-3876 [Aphanizomenon flos-aquae 2012/KM1/D3]
GTGGATAATGATAAAATGCAAGATAAAATTCCAACAATTGCAGAACTTCGAGAACTTTCTTTACGACTTCTTACAAAAAT
ACCTTACTTAAAAATGTTAGTTCTGTTTGGTTCTAGAGCAACAGGTAACATTAACGCCAATAGTGACTGGGATTTTGCTG
TTTTATATGATGAAGAAAAATATAATTTATACATTCAAAATAATCCATTAGCTGCATTTGTAATACCTGGAATTTTAGGA
GAAATATTCAAAATCAATTCTGACAAAATTGATATTGTAGAACTTAATCATTGTTCAAAACTCATAGCACATTTTGTAGC
TCGTGATGGAAAGGTATTATATGAAGAACCAGGAGATGAGTTTGATAAATTTCAACAAAGAGTTTTATTGAGTAATACAG
AAATTAAAAAGATTGAAAAAACTAAATTAGAAAACATTGAGAATTTTTTACAAAGGTGGGGAGTATGA
GTGGATAATGATAAAATGCAAGATAAAATTCCAACAATTGCAGAACTTCGAGAACTTTCTTTACGACTTCTTACAAAAAT
ACCTTACTTAAAAATGTTAGTTCTGTTTGGTTCTAGAGCAACAGGTAACATTAACGCCAATAGTGACTGGGATTTTGCTG
TTTTATATGATGAAGAAAAATATAATTTATACATTCAAAATAATCCATTAGCTGCATTTGTAATACCTGGAATTTTAGGA
GAAATATTCAAAATCAATTCTGACAAAATTGATATTGTAGAACTTAATCATTGTTCAAAACTCATAGCACATTTTGTAGC
TCGTGATGGAAAGGTATTATATGAAGAACCAGGAGATGAGTTTGATAAATTTCAACAAAGAGTTTTATTGAGTAATACAG
AAATTAAAAAGATTGAAAAAACTAAATTAGAAAACATTGAGAATTTTTTACAAAGGTGGGGAGTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Yingjie Song et al. (2023) Comparative analysis of five type II TA systems identified in Pseudomonas aeruginosa reveals their contributions to persistence and intracellular survival. Frontiers in Cellular And Infection Microbiology 13:1127786. [PubMed:36844395]
(2) Inga Songailiene et al. (2020) HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation. Molecular Cell 80(6):955-970.e7. [PubMed:33290744]