100301

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 517726..518290 Replicon chromosome
Accession NZ_CP027783
Organism Tetragenococcus osmophilus strain JCM 31126

Toxin (Protein)


Gene name mazF Uniprot ID A0A2H6CPR8
Locus tag C7K38_RS02490 Protein ID WP_014123850.1
Coordinates 517726..518061 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3G5FHJ2
Locus tag C7K38_RS02495 Protein ID WP_041591760.1
Coordinates 518054..518290 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7K38_RS02470 513193..513753 + 561 WP_014123854.1 hypothetical protein -
C7K38_RS02475 513803..514666 + 864 WP_061840067.1 glycine betaine ABC transporter substrate-binding protein -
C7K38_RS02480 514828..516156 - 1329 WP_012345024.1 ISL3 family transposase -
C7K38_RS02485 516901..517452 - 552 WP_069028691.1 GNAT family N-acetyltransferase -
C7K38_RS02490 517726..518061 - 336 WP_014123850.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C7K38_RS02495 518054..518290 - 237 WP_041591760.1 hypothetical protein Antitoxin
C7K38_RS02500 518793..520210 - 1418 Protein_486 ISLre2 family transposase -
C7K38_RS02505 520411..520473 + 63 Protein_487 hypothetical protein -
C7K38_RS02510 520528..521007 + 480 WP_094243845.1 UTRA domain-containing protein -
C7K38_RS02515 521276..521912 - 637 Protein_489 IS110 family transposase -
C7K38_RS02520 522178..522870 - 693 WP_005225225.1 Crp/Fnr family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 518825..520210 1385


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12890.98 Da        Isoelectric Point: 8.9118

>T100301 WP_014123850.1 NZ_CP027783:c518061-517726 [Tetragenococcus osmophilus]
MTNHYTPEKGDIVWIDFDPSTGMEIQKRRPALVISRGNFNTATKFAVVCPITSTEIDLPTRYSLSENYKTKGQIVIHQMK
SLDFNKRKIDFIEKMNPVDLKKIEQIIEFII

Download         Length: 336 bp

>T100301 NZ_CP027783:c518061-517726 [Tetragenococcus osmophilus]
ATGACTAATCACTATACACCAGAAAAAGGAGACATAGTGTGGATCGACTTTGATCCTTCAACTGGAATGGAAATTCAAAA
GCGACGGCCAGCTCTGGTAATTTCGCGCGGAAATTTTAATACGGCGACAAAATTTGCGGTGGTATGTCCTATTACATCCA
CTGAAATAGATTTACCAACTAGATATAGTCTTTCAGAAAATTATAAAACAAAAGGACAAATAGTCATCCATCAAATGAAA
TCTTTAGATTTCAATAAACGCAAAATTGACTTTATTGAGAAAATGAACCCTGTGGACTTGAAGAAAATAGAACAGATCAT
TGAGTTCATTATTTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8506.60 Da        Isoelectric Point: 4.0338

>AT100301 WP_041591760.1 NZ_CP027783:c518290-518054 [Tetragenococcus osmophilus]
MLKTKTRAQGRSIVVTLPAEDGATVFPGKEYLVSYGNDGTIVLIPKIEDPFANVTEGAFYESDVWEDMPAVGKEVLDD

Download         Length: 237 bp

>AT100301 NZ_CP027783:c518290-518054 [Tetragenococcus osmophilus]
ATGCTAAAGACAAAAACAAGGGCACAAGGTAGATCTATCGTGGTTACTTTACCAGCTGAGGATGGGGCAACAGTTTTTCC
TGGGAAAGAATATCTTGTTTCATACGGAAATGATGGAACTATTGTTTTGATACCTAAAATTGAAGATCCGTTTGCTAATG
TTACAGAAGGAGCTTTTTACGAATCGGATGTATGGGAAGATATGCCAGCAGTTGGTAAAGAGGTGCTGGATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H6CPR8

Antitoxin

Source ID Structure
AlphaFold DB A0A3G5FHJ2

References