Detailed information of TA system TA100299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 194201..194802
• Replicon: chromosome
• Accession: NZ_CP027783
• Organism: Tetragenococcus osmophilus strain JCM 31126

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: C7K38_RS01025
• Protein ID: WP_123933974.1
• Coordinates: 194201..194545 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: C7K38_RS01030
• Protein ID: WP_123933976.1
• Coordinates: 194539..194802 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7K38_RS01005	190299..190997	-	699	WP_123933968.1	response regulator transcription factor	-
C7K38_RS01010	191221..191965	+	745	Protein_197	cyclase family protein	-
C7K38_RS01015	192129..193208	-	1080	WP_123933970.1	LD-carboxypeptidase	-
C7K38_RS01020	193365..193958	+	594	WP_123933972.1	DJ-1/PfpI family protein	-
C7K38_RS01025	194201..194545	-	345	WP_123933974.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C7K38_RS01030	194539..194802	-	264	WP_123933976.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C7K38_RS01035	194888..195595	-	708	WP_123933978.1	hydroxyacylglutathione hydrolase	-
C7K38_RS01040	195761..196531	+	771	WP_028790115.1	DeoR/GlpR family DNA-binding transcription regulator	-
C7K38_RS01045	196524..196757	+	234	WP_123933980.1	HAD hydrolase-like protein	-
C7K38_RS01050	196904..198226	+	1323	WP_123933982.1	IS1380 family transposase	-
C7K38_RS01055	198486..199409	+	924	WP_123933984.1	sugar-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-101)

Antitoxin

• SpoVT-AbrB domain (12-53)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13007.87 Da
• Isoelectric Point: 5.7167

>T100299 WP_123933974.1 NZ_CP027783:c194545-194201 [Tetragenococcus osmophilus]
VVTQGDIFYVDFNPRRGHEQRSRRPAIALSHDMVQQTSNMTIVAPISSTKRDFPMYYSLESTKVIYGKVLLDQTIALDLQ
ARNVTEADIVDKVSKKELTEIIAIYKLLFSVDDE

Nucleotide

• Length: 345 bp

>T100299 NZ_CP027783:c194545-194201 [Tetragenococcus osmophilus]
GTGGTAACCCAGGGAGATATTTTTTATGTGGACTTTAATCCAAGACGAGGTCATGAACAGAGGAGTCGACGTCCAGCAAT
TGCTTTAAGCCACGATATGGTGCAGCAAACAAGTAATATGACAATTGTAGCCCCCATTAGCTCAACAAAACGTGATTTTC
CCATGTATTATTCTTTGGAAAGTACCAAAGTTATTTATGGAAAAGTCTTATTGGACCAAACAATTGCATTAGATCTACAG
GCAAGAAATGTAACGGAAGCAGACATTGTGGATAAGGTTTCTAAAAAAGAATTAACTGAGATCATTGCTATTTATAAGTT
ATTATTTAGCGTTGATGATGAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10041.20 Da
• Isoelectric Point: 5.0197

>AT100299 WP_123933976.1 NZ_CP027783:c194802-194539 [Tetragenococcus osmophilus]
MNNIKDQTRITQWGNSKATRIPSYIVKQLGLEDNQVLSVTIQNNSIILTPIKKQPTNIHDLFAGWQDDGQRDHELDWGKE
EGNELPW

Nucleotide

• Length: 264 bp

>AT100299 NZ_CP027783:c194802-194539 [Tetragenococcus osmophilus]
ATGAATAACATTAAAGATCAAACTCGTATTACACAGTGGGGAAATTCGAAAGCTACTCGAATCCCAAGTTATATTGTAAA
ACAATTAGGTTTAGAAGATAATCAAGTCTTATCTGTAACTATCCAAAATAACTCAATCATTTTAACACCTATAAAAAAAC
AGCCGACTAATATTCATGACCTTTTTGCAGGCTGGCAAGATGACGGACAACGTGATCATGAGTTAGATTGGGGGAAAGAA
GAAGGTAACGAATTACCGTGGTAA